Search for dissertations about: "Solid-phase DNA amplification"

Found 5 swedish dissertations containing the words Solid-phase DNA amplification.

2. 1. Novel planar and particle-based microarrays for point-of-care diagnostics

   Author : Gustav Svedberg; Helene Svahn Andersson; Jesper Gantelius; Fridtjof Lund-Johansen; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Planar microarrays; Particle-based microarrays; Point-of-care diagnostics; Colorimetric detection; Signal enhancement; Isothermal DNA amplification; Solid-phase DNA amplification; Biotechnology; Bioteknologi;

   Abstract : Point-of-care assays are easy-to-use, portable and inexpensive tests that canbe used to aid diagnostics by measuring levels of disease-specific moleculesin settings where access to advanced laboratory equipment and trainedpersonnel are limited, such as at the patient's bedside or in low resourceparts of developing countries. In order to achieve high multiplexingcapacities, such assays can be based on planar microarrays consisting ofspots immobilized on a flat surface or on particle-based microarrays basedon populations of encoded particles. READ MORE

4. 2. Improved Techniques for High-Throughput Molecular Diagnostics

   Author : Mario Curcio; KTH; []
   Keywords : DNA; Molecular Diagnostics;

   Abstract : The amount of information derived from sequencing the humangenome is leading to an exponential increase in the rate atwhich genes and genetic disorders are mapped and characterized.As a consequence, the demand for genetic testing is alsodramatically increasing. READ MORE

5. 3. Molecular Tools for Biomarker Detection

   Author : Lei Chen; Ulf Landegren; Niko Hildebrandt; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Rolling circle amplification; padlock probe;

   Abstract : The advance of biological research promotes the emerging of new methods and solutions to answer the biological questions. This thesis describes several new molecular tools and their applications for the detection of genomic and proteomic information with extremely high sensitivity and specificity or simplify such detection procedures without compromising the performance. READ MORE

6. 4. Molecular diagnostics of infectious diseases

   Author : Malin Nygren; KTH; []
   Keywords : ;

   Abstract : In DNA-based diagnostics, the polymerase chain reaction(PCR) is the most widely used DNA amplification method. Toenable both sensitive and specific detection of agents causinginfectious diseases, the PCR needs to becombined with methodsto prepare the clinical sample containing the genetic materialof the pathogen. READ MORE

7. 5. Retroviral and related nucleic acid processing enzymes expression and detection : Applications on clinical studies of HIV and HERVs

   Author : Raymond Awad; Amnon Hizi; Uppsala universitet; []
   Keywords : Medical sciences; Reverse transcriptase; endogenous retrovirus; deoxycytidine kinase; HIV; HERVS; MEDICIN OCH VÅRD; MEDICINE; MEDICIN; Medical Virology; medicinsk virologi;

   Abstract : Reverse transcriptase (RT), the expression and detection of the enzyme marking the family Retroviridae, has been studied. A new RT activity assay adapted to 96-well microtitre plates is described. It uses a non- radioactive bromodeoxyuridine triphosphate (BrdUTP) substrate in a solid phase format. The assay was highly sensitive. READ MORE