Search for dissertations about: "Solid-phase proximity ligation assay"

Showing result 1 - 5 of 10 swedish dissertations containing the words Solid-phase proximity ligation assay.

  2. 1. Solid-phase Proximity Ligation Assays : High-performance and multiplex protein analyses

    Author : Spyros Darmanis; Masood Kamali-Moghaddam; Ulf Landegren; Mathias Uhlen; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; proximity ligation assay; multiplexed; biomarkers; solid-phase; microparticles; cancer; sequencing; Molecular biology; Molekylärbiologi; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

    Abstract : Protein biomarkers circulating in blood hold the promise of improved diagnosis, prognosis and follow-up of treatment of disease via minimally invasive procedures. For the discovery and validation of such biomarkers, methods are needed that can facilitate parallel, highly specific and in-depth analysis of the blood proteome. READ MORE

  4. 2. Proximity Ligation and Barcoding Assays : Tools for analysis of proteins and protein complexes

    Author : Di Wu; Masood Kamali-Moghaddam; Ulf Landegren; Ola Söderberg; Jörg Hoheisel; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proximity ligation assay; proximity barcoding assay; sensitive; in situ; multiplex; profiling; amyloid-beta; MARK; tau; CSF; biomarker; protein complexes; Alzheimer’s disease; amyotrophic lateral sclerosis; Molekylär medicin; Molecular Medicine;

    Abstract : Proteins are fundamental structural, enzymatic and regulatory components of cells. Analysis of proteins, such as by measuring their concentrations, characterizing their modifications, and detecting their interactions, provides insights in how biological systems work physiologically or pathologically at the molecular level. READ MORE

  5. 3. Development and Application of Proximity Assays for Proteome Analysis in Medicine

    Author : Felipe Marques Souza de Oliveira; Masood Kamali-Moghaddam; Ulf Landegren; Mats Gullberg; Janne Lehtiö; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Solid-phase proximity ligation assay; post-translational modifications; glycosylation; phosphorylation; Enzyme-linked immunosorbent assay; immunoassay and rolling circle amplification; Proximity Extension Assay; inflammation protein biomarkers; autoantibodies; autoimmune disease;

    Abstract : Along with proteins, a myriad of different molecular biomarkers, such as post-translational modifications and autoantibodies, could be used in an attempt to improve disease detection and progression. In this thesis, I build on several iterations of the proximity ligation assay to develop and apply new adaptable methods to facilitate detection of proteins, autoantibodies and post-translational modifications. READ MORE

  6. 4. Proximity Ligation Assays for Disease Biomarkers Analysis

    Author : Rachel Yuan Nong; Ulf Landegren; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proximity ligation assay; blood biomarkers; protein interactions; pathway analysis; single molecule; next-generation sequencing; Molekylär medicin; Molecular Medicine;

    Abstract : One of the pressing needs in the field of disease biomarker discovery is new technologies that could allow high performance protein analysis in different types of clinical material, such as blood and solid tissues. This thesis includes four approaches that address important limitations of current technologies, thus enabling highly sensitive, specific and parallel protein measurements. READ MORE

  7. 5. Biomarkers for Better Understanding of the Pathophysiology and Treatment of Chronic Pain : Investigations of Human Biofluids

    Author : Anne-Li Lind; Torsten Gordh; Masood Kamali-Moghaddam; Jonas Bergquist; Eva Kosek; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; chronic pain; neuropathic pain; lumbar radicular pain; amyotrophic lateral sclerosis; radiculopathy; fibromyalgia; pathophysiology; spinal cord stimulation; mechanism of action; disc herniation; cerebrospinal fluid; plasma; biomarker; human; protein; chemokines; cytokines; inflammation; neuroinflammation; mass spectrometry; proximity ligation assay; proximity extension assay; antibody suspension bead array; protein profiling; molecular medicine; personalized medicine; Anestesiologi och intensivvård; Anaesthesiology and Intensive Care; Biomedical Laboratory Science; Biomedicinsk laboratorievetenskap; Kemi med inriktning mot analytisk kemi; Chemistry with specialization in Analytical Chemistry; Medicinsk vetenskap; Medical Science; Molekylär medicin; Molecular Medicine;

    Abstract : Chronic pain affects 20 % of the global population, causes suffering, is difficult to treat, and constitutes a large economic burden for society. So far, the characterization of molecular mechanisms of chronic pain-like behaviors in animal models has not translated into effective treatments. READ MORE