Search for dissertations about: "Super Resolution Microscopy"

Showing result 16 - 20 of 36 swedish dissertations containing the words Super Resolution Microscopy.

  1. 16. Advancing tissue clearing and expansion methods for high-resolution volumetric imaging of biological samples

    Author : Steven Edwards; Hjalmar Brismar; Etsuo Susaki; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biologisk fysik; Biological Physics;

    Abstract : The development of advanced light microscopes, capable of imaging samples at ever-higher spatial resolution and increasing speeds is an ongoing endeavour. The sample itself is an integral part of the microscope and, unlike the intricately positioned and highly polished lenses, it is an optically unpredictable component. READ MORE

  2. 17. Imaging Living Cells with an X-ray Laser

    Author : Gijs van der Schot; Tomas Ekeberg; Janos Hajdu; Filipe Maia; Pierre Thibault; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Coherent diffractive X-ray imaging; flash X-ray imaging; lensless imaging; single particle imaging; cyanobacteria; phasing; image classification; substrate-free sample delivery; X-ray free-electron laser; XFEL; FEL; CDXI; CDI; CXI; FXI; Chemistry with specialization in Biophysics; Kemi med inriktning mot biofysik;

    Abstract : Imaging living cells at a resolution higher than the resolution of optical microscopy is a significant challenge. Fluorescence microscopy can achieve a degree of super-resolution via labeling cellular components with a fluorescent dye. READ MORE

  3. 18. Investigation of Neuronal Protein Trafficking at the Molecular Scale

    Author : Martina Damenti; Ilaria Testa; Clive Bramham; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Endoplasmic Reticulum; Mitochondria; Arc Activity-regulated cytoskeleton-associated protein ; oligomerization; pRESOLFT; STED; event-triggered FCS; DNA-PAINT; STARSS; molecular dynamics simulations; Biological and Biomedical Physics; Biologisk och biomedicinsk fysik;

    Abstract : Neurons are polarized cells that encode information in the nervous system viaelectrochemical connections named synapses. The tuning of synapticconnections is enabled by a plastic protein trafficking system which operates atthe nanoscale to finely tweak the neuronal ultrastructure. READ MORE

  4. 19. Applications of DNA origami encoded nanoscale patterns

    Author : Ferenc Fördös; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : It was almost four decades ago when the recognition of DNA’s potential use as a programmable, self-assembling building material for nanostructures led to the birth and rapid expansion of the field of DNA nanotechnology, but it was two decades later when the development of the DNA origami technique initiated the widespread use of DNA based nanoconstructs through the simplification of the design process and the reduction of the required control over the quality and stoichiometry of the assembly components by using a single-stranded “scaffold” DNA and a set of “staple” oligonucleotides that “fold” the mentioned scaffold DNA into a predesigned shape by binding different regions of the scaffold strand together. This robust approach not only permitted the construction of intricate two- and three-dimensional structures, but it also allowed the design and fabrication of molecular patterns with unprecedented accuracy as each functionalizible component’s relative position in the DNA origami structure is known to nanometer precision. READ MORE

  5. 20. Membrane mechanisms during exo- and endocytosis in excitable cells

    Author : Gianvito Arpino; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : Excitable cells, like endocrine cells and neurons release hormones and transmitters through exocytosis to mediate many important functions, such as stress responses, immune responses, control of blood glucose, and synaptic transmission that mediates communication between neurons in the brain. This process relies on delicate endocytic mechanisms, to retrieve vesicular membranes and proteins previously fused at the plasma membrane and thus maintains the ability of exocytosis for excitable cells. READ MORE