Search for dissertations about: "Superporous agarose"

Found 4 swedish dissertations containing the words Superporous agarose.

  2. 1. Superporous agarose a new material for chromatography

    Author : Per-Erik Gustavsson; Tillämpad biokemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Protein separations; Chromatography; Superporous agarose; Continuous agarose beds; Intraparticle convection; Pore flow; Biochemistry; Metabolism; Biokemi; metabolism; Biotechnology; Bioteknik;

    Abstract : Agarose, the most commonly employed chromatography base material in industrial purification processes for biomolecules – proteins, has almost ideal properties. The major disadvantage of the agarose support is its poor mechanical strength which excludes its use in high speed separations where small particles are needed in order to achieve improved chromatographic efficiency. READ MORE

  4. 2. Novel Chromatography Matrices for Fast Isolation of Plasmid DNA

    Author : Peter Tiainen; Tillämpad biokemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biokemisk teknik; Polymer technology; biopolymers; Polymerteknik; Biotechnology; Bioteknik; Biochemical technology; Plasmid recovery.; Composite materials; Kompositmaterial; Coating and surface treatment; Skikt och ytbehandling; Superporous agarose; Silica fibres; Plasmid purification; Anion-exchange matrices;

    Abstract : As new therapeutic areas in which genetic material is employed, i.e. gene therapy and DNA vaccination, are expanding, a substantial increase in the demand for gene vectors, e.g. READ MORE

  5. 3. Fast isolation of proteins from bioreactors using affinity chromatography techniques

    Author : Eva Pålsson; Avdelningen för klinisk genetik; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; chromatography; protein purification; analyses; dispersion; chromatography matrices; Biochemistry; Biokemi; Metabolism; expanded bed; metabolism; Biotechnology; Bioteknik;

    Abstract : The purification of proteins from raw materials like fermentation broth and other biological sources usually involves several purification steps and is rather time consuming and expensive. This is a major concern in the production of many important protein products e.g. pharmaceuticals such as insulin and blood coagulation factors. READ MORE

  6. 4. Chromatographic Studies of Solute Interactions with Immobilized Red Blood Cells and Biomembranes

    Author : Ingo Gottschalk; Irving W. Wainer; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Affinity; Binding; Biomembrane; Biotin; Chromatography; Cytochalasin B; Dissociation constant; Drug absorption; Equilibrium; Glucose; GLUT1; Immobilization; Immobilized biomembrane affinity chromatography; Immobilized liposome chromatography; Interaction; Liposome; Membrane protein; Membrane vesicle; Partitioning; Phospholipid bilayer; Proteoliposome; Quantitative; Red blood cell; Solute; Specific; Streptavidin; Wheat germ agglutinin; Biokemi; Biochemistry; Biokemi; Biokemi; Biochemistry;

    Abstract : Specific and non-specific interactions of solutes with immobilized biomembranes were studied using chromatographic methods. Liposomes, proteoliposomes and red blood cell (RBC) membrane vesicles were immobilized by a freeze-thawing procedure, whereas whole RBCs were adsorbed in the gel beds using electrostatic interaction, binding to wheat germ agglutinin (WGA) or the streptavidin-biotin interaction. READ MORE