Search for dissertations about: "TIRF"

Showing result 1 - 5 of 17 swedish dissertations containing the word TIRF.

  1. 1. Adsorption of biopolymers and their layer-by-layer assemblies on hydrophilic surfaces

    Author : Maria Lundin; Eva Blomberg; Thomas Arnebrant; KTH; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Layer-by-layer; multilayer; adsorption; biopolymers; Chitosan; Heparin; Mucin; Albumin; Lysozyme; β-casein; SDS; QCM-D; Ellipsometri; DPI; TIRF; FRET; SFA; layer structure; solvent content; vertical diffusion; exponential growth; solid-liquid interface; deposition conditions; Surface and colloid chemistry; Yt- och kolloidkemi;

    Abstract : It is widely known that surfaces play an important role in numerous biological processes and technological applications. Thus, being able to modify surface properties provides an opportunity to control many phenomena occurring at interfaces. READ MORE

  2. 2. Caveolae associated proteins and how they effect caveolae dynamics

    Author : Björn Morén; Richard Lundmark; Volker Haucke; Umeå universitet; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Caveolae; caveolin; EHD2; cavin; microdomain; microscopy; TIRF; AFM; medicinsk biokemi; Medical Biochemistry;

    Abstract : Caveolae are a type of invaginated membrane domain that has been shown to be involved in several disease states, including lipodystrophy, muscular dystrophies and cancer. Several of these diseases are caused by the lack of caveolae or caveolae-related signaling deficiencies in the tissues in which the caveolar domain are abundant such as lung, adipose, muscle and their related endothelial cells. READ MORE

  3. 3. Modification of Surface Properties Through Polymer Adsorption. Kinetics, Interaction, Degradation and Stability

    Author : Dries Muller; Fysikalisk kemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TIRF.; protein; poly lactide ; poly ethylene oxide ; gelatin; exchange; enzyme; ellipsometry; desorption; degradation; adsorption; copolymers; Physical chemistry; Fysikalisk kemi;

    Abstract : The interfacial behaviour of polymers in relation to surface modification and polymer degradation was studied. In the first part the interfacial behaviour of amphiphilic poly(ethylene oxide)–poly(D,L-lactide) (EmLn) diblock copolymers at hydrophilic and hydrophobic substrates from aquous solution was studied. READ MORE

  4. 4. Characterization of artificial and biological lipid vesicles using TIRF and SPR

    Author : Thomas Olsson; Chalmers University of Technology; []
    Keywords : NATURVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; NATURAL SCIENCES; MEDICAL AND HEALTH SCIENCES; membrane permeability; chromaffin vesicle; fluorescence; total internal reflection TIRF microscopy; surface plasmon resonance SPR ; vesicle; osmotic shock; cell membrane; liposome; nanoparticle tracking analysis NTA ; large dense core vesicles LDCVs ;

    Abstract : Synthetic lipid vesicles serve as important mimics of cells and the natural membranes that they are enclosed by. As such they are frequently used as simplified models of the highly complex cell membrane to aid in-depth physicochemical and biological characterization of this essential biological structure. READ MORE

  5. 5. Assay development for studies of G protein-coupled receptors at the single-molecule level

    Author : Olov Wahlsten; Chalmers University of Technology; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; TIRF microscopy; colocalization; drug discovery; QCM-D; GPCR; pharmacodynamics; kinetics; equilibrium fluctuation analysis;

    Abstract : G protein-coupled receptors (GPCRs), also known as seven transmembrane (7TM) receptors,is the largest family of cell surface receptors. These receptors play a key role in transmittinga wide variety of signals across the cell membrane and are involved in physiologicalprocesses such as sensory transduction, cell-cell communication, neuronal transmission,and hormonal signaling. READ MORE