Search for dissertations about: "Transglycosylation"

Showing result 1 - 5 of 17 swedish dissertations containing the word Transglycosylation.

  1. 1. Transglycosylation catalyzed by β-mannanases : Molecular aspects relevant for enzymatic synthesis

    Author : Johan Morrill; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; β-mannanase; glycoside hydrolase; enzymology; kinetics; MALDI-TOF MS; HPLC; transglycosylation; alcoholysis; enzyme engineering; protein structure; enzymatic synthesis;

    Abstract : β-Mannanases are a major type of enzyme involved in the hydrolysis and modification of β-mannans, a hemicellulose present in high amounts in e.g. softwoods such as spruce. The β-mannanases studied in this thesis belong to glycoside hydrolase (GH) families GH5 and GH26. READ MORE

  2. 2. β-Mannoside hydrolases from clan A – Structural enzymology, subsite engineering and transglycosylation

    Author : Anna Rosengren; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; β-mannosidase; β-mannanase; enzymology; kinetics; MALDI-TOF MS; protein engineering; protein structure; subsite organisation; transglycosylation;

    Abstract : β-Mannoside hydrolases are enzymes that are involved in conversion of the major soft-wood hemicellulose O-acetyl-galactoglucomannan, an important renewable biomass resource. They belong to clan A of glycoside hydrolases and share a common (β/α)8-TIM barrel fold. READ MORE

  3. 3. Structure and Function of Beta-Mannanases from Glycoside Hydrolase Clan A. A Study of Hemicellulases from Microbes and a Mollusc

    Author : Lars Anderson; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Proteiner; enzymologi; enzymology; Proteins; subsite organisation; transglycosylation; enzyme kinetics; beta-mannanase; hemicellulose;

    Abstract : Hemicellulose is the second most abundant group of renewable polysaccharides. Mannans can be found as the major hemicellulose in softwood and as storage polysaccharides in a range of plants. In this work, structural and functional characterisations of family 5 and 26 endo-1,4-b-mannanases (b-mannanases) have been performed. READ MORE

  4. 4. Heterologous expression and characterization of xyloglucan endotransglycosylases from Populus tremula x tremuloides

    Author : Åsa Kallas; KTH; []
    Keywords : xyloglucan endotransglycosylase; transglycosylation; hybrid aspen; cell expansion; heterologous expression; Pichia pastoris; N-glycosylation; X-ray crystallography;

    Abstract : Xyloglucan endotransglycosylases (XETs) are enzymes involvedin the reorganization of the primary cell wall. This thesisdescribes several aspects of XET16A from hybrid aspen (Populustremula x tremuloides). The gene was isolated from the hybridaspen cDNA library and the protein was expressed heterologouslyin the methylotrophic yeast Pichia pastoris. READ MORE

  5. 5. galactmannan degradation by fungi and gut bacteria : structural enzymology and fine -tuned substrate specifcity

    Author : Sumitha Krishnaswamyreddy; Biokemi och Strukturbiologi; []
    Keywords : fine-tuned substrate specificity; β-mannanase; β-mannosidases; synergy; α-galactosidases; mannan converison in gut; transglycosylation; polysaccharide utilisation locus; phylogenetic analysis; MALDI-TOF MS;

    Abstract : AbstractThe degradation of plant based β-mannan polysaccharides represents one of the many challenges efficiently tackled by microorganisms living in different habitats. In this thesis, glycoside hydrolases (GHs) involved in mannan degradation from two different organisms, Aspergillus nidulans (paper I and II) and Bacteroides ovatus (paper III and IV) were studied. READ MORE