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Showing result 1 - 5 of 19 swedish dissertations matching the above criteria.

2. 1. Transglycosylation catalyzed by β-mannanases : Molecular aspects relevant for enzymatic synthesis

   Author : Johan Morrill; Biokemi och Strukturbiologi; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; β-mannanase; glycoside hydrolase; enzymology; kinetics; MALDI-TOF MS; HPLC; transglycosylation; alcoholysis; enzyme engineering; protein structure; enzymatic synthesis;

   Abstract : β-Mannanases are a major type of enzyme involved in the hydrolysis and modification of β-mannans, a hemicellulose present in high amounts in e.g. softwoods such as spruce. The β-mannanases studied in this thesis belong to glycoside hydrolase (GH) families GH5 and GH26. READ MORE

4. 2. β-Mannoside hydrolases from clan A – Structural enzymology, subsite engineering and transglycosylation

   Author : Anna Rosengren; Biokemi och Strukturbiologi; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; β-mannosidase; β-mannanase; enzymology; kinetics; MALDI-TOF MS; protein engineering; protein structure; subsite organisation; transglycosylation;

   Abstract : β-Mannoside hydrolases are enzymes that are involved in conversion of the major soft-wood hemicellulose O-acetyl-galactoglucomannan, an important renewable biomass resource. They belong to clan A of glycoside hydrolases and share a common (β/α)8-TIM barrel fold. READ MORE

5. 3. Enzymatic conversion of β-mannans: Analysing, evaluating and modifying transglycosylation properties of glycoside hydrolases

   Author : Mathias Wiemann; Biokemi och Strukturbiologi; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; beta-mannanase; alpha-galactosidase; enzyme engineering; transglycosylation; MALDI-TOF MS; HPLC;

   Abstract : Retaining glycoside hydrolases are enzymes that catalyse the breakdown down of glycans through hydrolysis. Due to the double-replacement mechanism of the retaining glycoside hydrolases (GHs), which form an intermediate with part of the glycan covalently attached to the enzyme, some GHs are able to catalyse synthesis reactions called transglycosylation. READ MORE

6. 4. Structure and Function of Beta-Mannanases from Glycoside Hydrolase Clan A. A Study of Hemicellulases from Microbes and a Mollusc

   Author : Lars Anderson; Biokemi och Strukturbiologi; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Proteiner; enzymologi; enzymology; Proteins; subsite organisation; transglycosylation; enzyme kinetics; beta-mannanase; hemicellulose;

   Abstract : Hemicellulose is the second most abundant group of renewable polysaccharides. Mannans can be found as the major hemicellulose in softwood and as storage polysaccharides in a range of plants. In this work, structural and functional characterisations of family 5 and 26 endo-1,4-b-mannanases (b-mannanases) have been performed. READ MORE

7. 5. Exploring beta-mannan acting glycosidase families in hydrolysis and mannosyl transfer

   Author : Simon Birgersson; Biokemi och Strukturbiologi; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; glycoside hydrolases; beta-mannanase; Transglycosylation; enzymatic synthesis; Enzyme synergy; enzyme engineering; MALDI-TOF; HPLC; NMR;

   Abstract : The thesis revolves around the study of glycoside hydrolases (GH), specifically GH5 β-mannanases and GH36 α-galactosidase (I-III), as well as a GH130 mannoside phosphorylase (I, IV). These enzymes are investigated for their actions on and conversion of hemicellulosic β-mannan, including food-grade galactomannan and softwood galactoglucomannan, along with manno-oligosaccharides (MOS) derived from such polymers. READ MORE