Search for dissertations about: "Unnatural amino acid"
Showing result 1 - 5 of 10 swedish dissertations containing the words Unnatural amino acid.
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1. Exotic Ribosomal Enzymology
Abstract : This thesis clarifies intriguing enzymology of the ribosome, the multiRNA/multiprotein complex that catalyzes protein synthesis (translation). The large ribosomal RNAs (23S and 16S rRNAs in E. coli) are post-transcriptionally modified by many specific modification enzymes, yet the functions of the modifications remain enigmatic. READ MORE
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2. Rate and Accuracy of Bacterial Protein Synthesis with Natural and Unnatural Amino Acids
Abstract : This thesis addresses different questions regarding the rate, efficiency, and accuracy of peptide bond formation with natural as well as unnatural amino acids: Which step is rate-limiting during peptide bond formation? How does the accuracy vary with different transfer RNAs (tRNAs) and codons and how is it relevant to the living cells? Does proofreading selection of codon reading occur in a single- or multi-step manner as theoretically suggested? How does the E. coli translation system discriminate unnatural amino acids? Based on that, how to improve the incorporation efficiencies of unnatural amino acids?Based on the study on pH dependence of peptide bond formation, we show that the rate of the chemistry of peptidyl transfer to aminoacyl-tRNA (AA-tRNA) Gly-tRNAGly or Pro-tRNAPro limits the rate of peptide bond formation at physiological pH 7. READ MORE
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3. Structure-Function Relationships of Pi Class Glutathione Transferase Studied by Protein Engineering
Abstract : The glutathione transferases (GSTs) represent a superfamily of dimeric proteins involved in cellular detoxication by catalyzing the nucleophilic addition of the reduced glutathione (GSH) to the hydrophobic electrophiles. The present work focuses on the functional role of the conserved structures of GSTP1-1. READ MORE
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4. Synthesis of Non-Natural Aromatic Amino Acids Derivatives Solvent-free reactions-Versatile building blocks-Galectin inhibitors
Abstract : This thesis deals with three topics concerning the synthesis of non-natural also known as unnatural amino acid derivatives. In the first, solvent-free reaction conditions for the Heck reaction were developed that were superior to previous conditions because the reaction time could be reduced and the work-up procedure simplified. READ MORE
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5. Palladium(0)-Catalyzed Synthesis of Spirocycles and Supercritical Chemistry using a Resistively Heated Flow Reactor
Abstract : This doctoral thesis focusses on an effective and selective approach to the synthesis of spirocycles using palladium(0)-catalyzed Mizoroki-Heck reactions. In addition, selective and efficient chemistry was highlighted by the design and evaluation of a novel resistively heated system for continuous flow (CF) synthesis for high-temperature and high-pressure applications. READ MORE