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Found 2 swedish dissertations matching the above criteria.

2. 1. Towards Understanding of Selectivity & Enantioconvergence of an Epoxide Hydrolase

   Author : Åsa Janfalk Carlsson; Mikael Widersten; Uwe T. Bornscheuer; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Epoxide hydrolase; Epoxide; Enantioselectivity; Regioselectivity; Enantioconvergence; Crystal structures; Biocatalysis; Immobilization; Transient kinetics; CASTing; Directed evolution; Biokemi; Biochemistry;

   Abstract : Epoxide hydrolase I from Solanum tuberosum (StEH1) and isolated variants thereof has been studied for mapping structure-function relationships with the ultimate goal of being able to in silico predict modifications needed for a certain activity or selectivity. To solve this, directed evoultion using CASTing and an ISM approach was applied to improve selectivity towards either of the enantiomeric product diols from (2,3-epoxypropyl)benzene (1). READ MORE

4. 2. Protein Engineering of Candida antarctica Lipase A : Enhancing Enzyme Properties by Evolutionary and Semi-Rational Methods

   Author : Anders G. Sandström; Jan-Erling Bäckvall; Uwe T. Bornscheuer; Stockholms universitet; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; lipase; protein engineering; directed evolution; kinetic resolution; structural biology; Enzyme engineering; Enzymteknik; organisk kemi; Organic Chemistry;

   Abstract : Enzymes are gaining increasing importance as catalysts for selective transformations in organic synthetic chemistry. The engineering and design of enzymes is a developing, growing research field that is employed in biocatalysis. READ MORE