Search for dissertations about: "Vitreoscilla hemoglobin VHb"

Found 5 swedish dissertations containing the words Vitreoscilla hemoglobin VHb.

2. 1. Vitreoscilla hemoglobin. In vitro mutagenesis and transcript profiling to improve its biotechnological usefulness

   Author : Charlotte Andersson; Tillämpad biokemi; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemical technology; Biokemisk teknik; gene expression analysis; transcript profiling; microarray; DNA chips; microaerobic; hypoxia; oxygen-limited; bacterial hemoglobin; Escherichia coli; Vitreoscilla hemoglobin VHb ;

   Abstract : Vitreoscilla is an obligate aerobic bacterium found in oxygen-poor environments such as stagnant ponds and decaying vegetable matter. Vitreoscilla induces the production of a homodimeric hemoglobin (VHb) when subjected to oxygen-limiting conditions. READ MORE

4. 2. Ribosomes and subunits from Escherichia coli studied by asymmetrical flow field-flow fractionation

   Author : Mikael Nilsson; Centrum för analys och syntes; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; metabolic engineering; VHb; Vitreoscilla hemoglobin; E. coli; Escherichia coli; separation; subunits; ribosomes; Asymmetrical flow field-flow fractionation; flow FFF; optimization; biotechnological production; Analytical chemistry; Analytisk kemi;

   Abstract : In this thesis asymmetrical flow field-flow fractionation (FFF) separations of ribosomes and subunits have been studied. Ribosomal material was prepared from Escherichia coli (E. coli) cells and analyzed by the size separation technique. READ MORE

5. 3. Vitreoscilla Haemoglobin. Genetic Fusions and Metabolic Characterisation

   Author : Viktoria Roos; Tillämpad biokemi; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Metabolism; Biokemi; metabolism; osmotic stress; transcript profiling; DNA microarrays; random elongation mutagenesis; directed evolution; mRNA structure; Vitreoscilla haemoglobin VHb ; Escherichia coli; salt stress.;

   Abstract : Vitreoscilla is an obligate aerobic bacterium living in oxygen-poor environments such as stagnant ponds and decaying vegetable matter. Vitreoscilla expresses a homodimeric haemoglobin (VHb) when subjected to oxygen-limited conditions. READ MORE

6. 4. Vitreoscilla Haemoglobin - Peroxidase Activity and Metabolic Aspects

   Author : Malin Kvist; Tillämpad biokemi; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; metabolism; Biokemi; Metabolism; Biochemistry; Naturvetenskap; Natural science; lipid peroxidation; hypoxia; salt stress; Escherichia coli; Lactococcus lactis; hydrogen-bonding network; proximal site; mutations; Vitreoscilla haemoglobin; peroxidase activity; Proteins; enzymology; Proteiner; enzymologi;

   Abstract : The function of non-vertebrate haemoglobins (Hbs) appears more diverse than that of vertebrate Hbs, possibly reflecting the various demands to be met among their hosts. Sharing structural features with other haem proteins/enzymes, Hbs display a qualitative overlap in activities with functions extending beyond oxygen transport/storage. READ MORE

7. 5. An Investigation of Peroxidase Activity in Biomimetic and Biological Systems

   Author : Ekaterina Ryabova; Kemiska institutionen; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; metabolism; Metabolism; enzymologi; Proteiner; enzymology; Proteins; hemoglobin; catalysis; peptide; peroxidase; protoporphyrin IX; Biokemi; Biochemistry;

   Abstract : In order to create a heme environment which permits biomimicry of heme-containing plant peroxidases and investigate the role played by the vinyl side chains of the porphyrin ring on the stability and, possibly, catalytic activity of model compounds, a number of new synthetic microperoxidases -– hemin-6(7)-gly-his methyl ester (HGH) and hemin-6(7)-gly-gly-his methyl ester (HGGH) and hemin-6,7-bis(gly-gly-his methyl ester) (H2GGH) as well as mesohemin-6(7)-gly-gly-his methyl ester (MGGH) and deuterohemin-6(7)-gly-gly-his methyl ester (DGGH) have been prepared by condensation of a peptide residue with the propionic side chains of hemin, mesohemin and deuterohemin, respectively. Reactivity studies towards different organic and inorganic substrates showed that not only five- but also six-coordinate hemin peptide complexes catalyze the oxidation of substrates by H2O2. READ MORE