Search for dissertations about: "XTH"

Found 4 swedish dissertations containing the word XTH.

  2. 1. Plant and microbial xyloglucanases: Function, Structure and Phylogeny

    Author : Jens Eklöf; Harry Brumer; Harry Gilbert; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; xyloglucan; xyloglucanases; XTH; XET; XEH; endoglucanases; EG16; Molecular biology; Molekylärbiologi;

    Abstract : In this thesis, enzymes acting on the primary cell wall hemicellulose xyloglucan are studied.  Xyloglucans are ubiquitous in land plants which make them an important polysaccharide to utilise for microbes and a potentially interesting raw material for various industries. READ MORE

  4. 2. In vitro and in vivo approaches in the characterization of XTH gene products

    Author : Nomchit Kaewthai; Harry Brumer; Jocelyn Rose; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Arabiodopsis thaliana; Hordeum vulgare; plant cell wall; xyloglucan; glycoside hydrolase family 16; xyloglucan endo-transglycosylase hydrolase gene family; xyloglucan endo-hydrolase; heterologous protein expression; Pichia pastoris; T-DNA insertion; in situ XET XEH assay; high-througput screening; Plant physiology; Växtfysiologi; Biochemistry; Biokemi; Functional genomics; Funktionsgenomik; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

    Abstract : ABSTRACT The xyloglucan endo-transglycosylase/hydrolase (XTH) genes are found in all vascular and some nonvascular plants. The XTH genes encode proteins which comprise a subfamily of glycoside hydrolase (GH) family 16 in the Carbohydrate-Active enZYmes (CAZY) classification. READ MORE

  5. 3. On the engineering of proteins: methods and applications for carbohydrate-active enzymes

    Author : Fredrika Gullfot; Harry Brumer; Antoni Planas; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; enzyme engineering; rational design; directed evolution; DNA shuffling; glycosynthase; xyloglucan; xyloglucan endo-transglycosylase; retaining glycoside hydrolase; xyloglucanase; carbohydrate binding module; polysaccharide synthesis; Bioengineering; Bioteknik;

    Abstract : This thesis presents the application of different protein engineering methods on enzymes and non-catalytic proteins that act upon xyloglucans. Xyloglucans are polysaccharides found as storage polymers in seeds and tubers, and as cross-linking glucans in the cell wall of plants. READ MORE

  6. 4. A holistic approach to understanding CAZy families through reductionist methods

    Author : Jens Eklöf; Harry Brumer; Per-Åke Nygren; Lars Arvestad; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Carbohydrate esterase family 8; XET; PME; YbhC; Biochemistry; Biokemi;

    Abstract :   In a time when the amount of biological data present in the public domain is becoming increasingly vast, the need for good classification systems has never been greater. In the field of glycoscience the necessity of a good classification for the enzymes involved in the biosynthesis, modification and degradation of polysaccharides is even more pronounced than in other fields. READ MORE