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Showing result 1 - 5 of 18 swedish dissertations matching the above criteria.

  2. 1. The GRE3 encoded aldo-keto reductase and its influence on xylose fermentation in recombinant Saccharomyces cerevisiae strains

    Author : Karin Träff; Teknisk mikrobiologi; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biochemical technology; Biokemisk teknik; dihydroxyacetone phosphate; xylose reductase; xylose; xylose isomerase; GRE3; Saccharomyces cerevisiae; aldo-keto reductase;

    Abstract : The aim of the work presented in this thesis was to improve ethanol formation from xylose in recombinant Saccharomyces cerevisiae strains. This was achieved by decreasing the xylitol by-product formation. Gre3p is the major xylose-reducing enzyme in S. cerevisiae. READ MORE

  4. 2. Genetic Traits Beneficial for Xylose Utilization by Recombinant Saccharomyces cerevisiae

    Author : Oskar Bengtsson; Teknisk mikrobiologi; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Saccharomyces cerevisiae; metabolic engineering; fermentation; xylose; ethanol; promoter; xylose reductase; cofactor; validation; transcriptome;

    Abstract : Saccharomyces cerevisiae ferments hexoses in lignocellulosic hydrolysates under anaerobic conditions with high rates and ethanol yields. However, S. cerevisiae is naturally unable to utilize the pentose fraction of the hydrolysates. Xylose is the most abundant pentose sugar, and although recombinant S. READ MORE

  5. 3. Redox balancing in recombinant strains of Saccharomyces cerevisiae

    Author : Mikael Anderlund; Teknisk mikrobiologi; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; xylose isomerase; xylitol dehydrogenase; xylose reductase; glycerol; xylitol; xylose; E. coli; A. vinelandii; T. thermophilus; S. cerevisiae; P. stipitis; transhydrogenase.; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Abstract : In metabolically engineered Saccharomyces cerevisiae expressing Pichia stipitis XYL1 and XYL2 genes, encoding xylose reductase (XR) and xylitol dehydrogenase (XDH), respectively, xylitol is excreted as the major product during anaerobic xylose fermentation and only low yields of ethanol are produced. This has been interpreted as a result of the dual cofactor dependence of XR and the exclusive use of NAD+ by XDH. READ MORE

  6. 4. Metabolic Control Points in Ethanolic Fermentation of Xylose by Saccharomyces cerevisiae

    Author : David Runquist; Teknisk mikrobiologi; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Transcriptomics; Xylose reductase; Saccharomyces cerevisiae; Xylose; Ethanol; Transport; Lignocellulose;

    Abstract : Baker’s yeast, Saccharomyces cerevisae, has been engineered to utilize the pentose sugar xylose present in lignocellulose biomass for the production of ethanol. Currently ethanol production from xylose is slow which limits the implementation of said strains in industrial production. READ MORE

  7. 5. Improving initial xylose metabolism in recombinant Saccharomyces cerevisiae

    Author : Márk Gárdonyi; Teknisk mikrobiologi; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biokemisk teknik; Biochemical technology; xylose isomerase; xylose transport; xylose fermentation; Saccharomyces cerevisiae; metabolic engineering;

    Abstract : The aim of the thesis is to improve the initial steps of xylose metabolism in recombinant Saccharomyces cerevisiae. S. cerevisiae takes up xylose of poor affinity by means of hexose transporters. Metabolic control analysis was used to investigate whether the low xylose utilisation rate is due to inefficient transport. READ MORE