Search for dissertations about: "affinity chromatography"

Showing result 6 - 10 of 128 swedish dissertations containing the words affinity chromatography.

  2. 6. Development of low affinity techniques for applications in clinical chemistry

    Author : Lisa Leickt; Avdelningen för klinisk kemi och farmakologi; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; chromatography performance; acute myocardial infarction; antibodies; creatine kinase MB isoforms; lectins; low affinity interactions; weak affinity chromatography; Clinical chemistry; Klinisk kemi;

    Abstract : An increase in the plasma level of creatine kinase MB (CKMB) has long been used to indicate acute myocardial infarction (AMI). For optimal treatment an early diagnosis is important. An increase in the ratio between the two isoforms of CKMB is seen much earlier than an increase in the total level of CKMB. READ MORE

  4. 7. Polymer Displacement and Supermacroporous Cryogels - Two New Tools in Chromatographic Separation of Biomolecules and Bioparticles

    Author : Pär Arvidsson; Bioteknik; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; biopolymers; Polymerteknik; Biochemical technology; Polymer technology; IMAC; dye-affinity; affinity chromatography; viable cells; cell chromatography; non-clarified cell homogenate; cryogel; Polymer displacement; supermacroporous; Biokemisk teknik;

    Abstract : Production of protein is often accompanied with difficulties due to the large amounts of different biological material resulting from fermentation. This makes purification necessary and affinity chromatography is usually involved as being biospecific, although suffering from limitations as low recovery due to ineffective elution and inability to handle particulate materials. READ MORE

  5. 8. Engineering Proteinaceous Ligands for Improved Performance in Affinity Chromatography Applications

    Author : Susanne Gülich; KTH; []
    Keywords : affinity chromatography; cleaning-in-place; deamidation; destabilization; linker engineering; protein engineering; stabilization; staphylococcal protein A; streptococcal protein G; turn loop engineering;

    Abstract : .... READ MORE

  6. 9. Carbohydrate-specific, boronate-containing copolymers: from sugar-sensing to cell adhesion

    Author : Marina Kuzimenkova; Bioteknik; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; surface grafting; chemisorption; boronic acid; carbohydrates; affinity; chromatography; smart polymers; biomimetics; hydrogels; molecular recognition; diffusion; sugar-sensing; cell adhesion;

    Abstract : Carbohydrates play a major role in many cell recognition events and metabolic pathways of living organisms. Synthetic carbohydrate receptors are of great interest for the chemical design of sugar sensors, materials for cell separation, cell immobilization, drug delivery and other biomedical/biotechnological applications. READ MORE

  7. 10. Introducing weak affinity chromatography to drug discovery with focus on fragment screening

    Author : Minh-Dao Duong-Thi; Sten Ohlson; Brian Dymock; Linnéuniversitetet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; affinity LC-MS; fragment-based drug discovery; fragment screening; high throughput; mass spectrometry; stereoisomer; enantiomer; thrombin; weak affinity chromatography; WAC; WAC-MS.; Biomedical Sciences; Biomedicinsk vetenskap; Biokemi; Biochemistry;

    Abstract : Fragment-based drug discovery is an emerging process that has gained popularity in recent years. The process starts from small molecules called fragments. One major step in fragment-based drug discovery is fragment screening, which is a strategy to screen libraries of small molecules to find hits. READ MORE