Search for dissertations about: "affinity protein"

Showing result 21 - 25 of 734 swedish dissertations containing the words affinity protein.

  2. 21. Engineering of small IgG binding domains for antibody labelling and purification

    Author : Sara Kanje; Sophia Hober; Cecília Roque; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Antibody; labelling; purification; Protein G; Protein A; protein engineering; protein design; combinatorial selection; Bioteknologi; Biotechnology;

    Abstract : In protein engineering, rational design and selection from combinatorial libraries are methods used to develop proteins with new or improved features. A very important protein for the biological sciences is the antibody that is used as a detecting agent in numerous laboratory assays. READ MORE

  4. 22. Selection of antigens for antibody-based proteomics

    Author : Lisa Berglund; Mathias Uhlén; Sören Brunak; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; epitope; antigen; antibody; affinity; protein; proteome; proteomics; bioinformatics; prediction; primer design; sequence similarity; Bioengineering; Bioteknik;

    Abstract : The human genome is predicted to contain ~20,500 protein-coding genes. The encoded proteins are the key players in the body, but the functions and localizations of most proteins are still unknown. READ MORE

  5. 23. Combinatorial protein engineering applied to enzyme catalysis and molecular recognition

    Author : Malin Eklund; KTH; []
    Keywords : phage display; selection; enzyme; affinity domains; crystal structure; affibody; lipase; protein engineering; protein A; assembly;

    Abstract : The recent development of methods for constructing andhandling large collections (libraries) of proteins, from whichvariants with desired traits can be isolated, hasrevolutionized the field of protein engineering. Key elementsof such methods are the various ways in which the genotypes(the genes) and the phenotypes (the encoded proteins) arephysically linked during the process. READ MORE

  6. 24. Antibody based plasma protein profiling

    Author : Ulrika Qundos; Jochen M. Schwenk; Peter Nilsson; Mathias Uhlén; Ulrike Korf; KTH; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; protein profiling; plasma; antibody; affinity proteomics; biomark- ers; multiplex; assay development;

    Abstract : This thesis is about protein profiling in serum and plasma using antibody suspension bead arrays for the analysis of biobanked samples and in the context of prostate cancer biomarker discovery. The influence of sample preparation methods on antibody based protein profiles were investigated (Papers I-III) and a prostate cancer candidate biomarker identified and verified (Papers III-V). READ MORE

  7. 25. Angiopoietin-like protein 4 : an unfolding chaperone regulating lipoprotein lipase activity

    Author : Valentina Sukonina; Gunilla Olivecrona; Lena Kjellen; Umeå universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Lipoprotein lipase; angiopoietin-like protein 4; protein folding; human adipose tissue; macrophages; peroxisome proliferator-activated receptor delta PPARδ ; Medical microbiology; Medicinsk mikrobiologi;

    Abstract : Lipoprotein lipase (LPL) is the main enzyme hydrolyzing triglyceride-rich lipoproteins in plasma. Proteoglycan-bound LPL on the vascular endothelium represent the functional pool of active enzyme. LPL is regulated in a tissue specific manner according to metabolic demands. READ MORE