Search for dissertations about: "affinity proteins"

Showing result 1 - 5 of 508 swedish dissertations containing the words affinity proteins.

  1. 1. Affinity Precipitation of Proteins. Characterization of Some Underlying Mechanisms

    Author : Eva Linné Larsson; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Purification; Proteins; Lectins; Eudragit S-100; Enzymes; Dynamic laser light scattering; Concanavalin A; Complex dissociation; Complex formation; Affinity precipitation; Alginate; Reversibly soluble-insoluble polymers; Rheology; Soya bean hemagglutinin; Biotechnology; Bioteknik;

    Abstract : Two polymers, alginate and Eudragit S-100 were used as reversibly soluble-insoluble ligand carriers for the selective isolation of enzymes and lectins respectively. These two polymers have the inherent property of being precipitated under well defined conditions. READ MORE

  2. 2. Metal Binding Tags - Characterisation, Use in Bioseparation and Applications of Green Fluorescent Fusion Proteins

    Author : Florent Bernaudat; Tillämpad biokemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Proteiner; enzymologi; enzymology; E. coli; Immobilised metal ion affinity chromatography; Affinity tags; Green fluorescent protein; Surface plasmon resonance; Genetic engineering; Screening; Proteins; Immunoassay; Biotechnology; Bioteknik;

    Abstract : Most of the recombinant proteins produced nowadays are fused to affinity tags, in order to facilitate their purification through affinity chromato-graphy. Out the different tags available, poly-histidine tags are among the most commonly used and can help purification through immobilised metal ion affinity chromatography (IMAC). READ MORE

  3. 3. On the application and validation of multiplexed affinity assays

    Author : Tea Dodig-Crnković; Jochen M. Schwenk; Mun-Gwan Hong; Peter Nilsson; Norman Leigh Anderson; KTH; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; Affinity proteomics; Antibody; Autoantibody; Multiplexed assays; Protein microarray; Plasma proteins; Suspension bead array; Biotechnology; Bioteknologi;

    Abstract : Proteins are essential macromolecules that carry out complex functions in human cells, tissues, and organs. They regulate a diverse set of biological processes and protect against pathogens. However, dysregulation or malformation of proteins can cause disease. READ MORE

  4. 4. Selective extraction systems for detection and purification of membrane proteins

    Author : Ulf Sivars; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; partitioning; Aqueous two-phase systems; separation; membrane proteins; detergent; micelle; polymer; Biochemistry; affinity; Metabolism; Biokemi;

    Abstract : Increased understanding of membrane proteins is important for characterization of biochemical processes. Improved membrane protein isolation methods is a key issue for effective functional and structural determination of the large amount of unknown membrane proteins. READ MORE

  5. 5. Generation and engineering of ABD-derived affinity proteins for clinical applications

    Author : Sarah Lindbo; Sophia Hober; Christoph Rader; KTH; []
    Keywords : ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; ABD; ADAPT; affinity proteins; protein engineering; radionuclide molecular imaging; HER2; Biotechnology; Bioteknologi;

    Abstract : Proteins that specifically recognize and bind to other molecules or structures are important tools in industrial and medical applications. Binding proteins engineered from small stable scaffold proteins have been utilized for several purposes due to their favorable biophysical properties, tolerance to mutagenesis, efficient tissue penetration and ease of production. READ MORE