Search for dissertations about: "affinity tags"

Showing result 1 - 5 of 22 swedish dissertations containing the words affinity tags.

  2. 1. Metal Binding Tags - Characterisation, Use in Bioseparation and Applications of Green Fluorescent Fusion Proteins

    Author : Florent Bernaudat; Tillämpad biokemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Proteiner; enzymologi; enzymology; E. coli; Immobilised metal ion affinity chromatography; Affinity tags; Green fluorescent protein; Surface plasmon resonance; Genetic engineering; Screening; Proteins; Immunoassay; Biotechnology; Bioteknik;

    Abstract : Most of the recombinant proteins produced nowadays are fused to affinity tags, in order to facilitate their purification through affinity chromato-graphy. Out the different tags available, poly-histidine tags are among the most commonly used and can help purification through immobilised metal ion affinity chromatography (IMAC). READ MORE

  4. 2. Genetically Engineered Plants: Improved stress tolerance, growth and facilitated protein purification

    Author : Gösta Lilius; Tillämpad biokemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; glycine betaine; gene; freezing; flooding; drought; dicoteledones; desiccation; compatible solutes; cold; choline dehydrogenase; chill; CDH; betaine; betA; alkaloids anoxia; affinity purification; affinity tags; handles; hemoglobin; hypoxia; low temperatu; Biotechnology; Bioteknik;

    Abstract : Heterologous genes were expressed in plants in which the overall goal was to increase the value of the final crop. Various aspects were explored and evaluated, such as improving the stress tolerance, enhancing the availability of oxygen in growing cells and producing rare proteins with increased degrees of purity. READ MORE

  5. 3. Transcriptome and Proteome Analysis using Signature Tags

    Author : Charlotta Agaton; KTH; []
    Keywords : functional genomics; 3´-end signature tags; pyrosequencing; amplification; PrEST; chromosome 21; polyclonal antibodies; dual expression; affinity purification;

    Abstract : With the full sequence of the human genome now available, anexciting era in biomedical research has started. The sequenceprovides information about all our genes and greatly increasesthe scope to compare genetic activities in different cells, toanalyze genetic variation between individuals and betweendifferent species and, most importantly, to investigatesystematically the whole genome in a gene-by-gene manner, andthus increase our understanding of gene function. READ MORE

  6. 4. Metal Affinity Peptides in Biotechnological Applications As tools in protein purification, protein stabilisation, metal quantification and metal tolerance of bacteria and plants

    Author : Malin Mejàre; Tillämpad biokemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; cadmium; metal quantification; fusion tag; metal affinity tag; phage display; peptide library; metal tolerance; metal resistance; metal accumulation; bioremediation; transgenic plant; tobacco; potato; protein purification; immobilised metal affinity chromatography; metal affinity precipitation; metal detection; protein stabilisation; Bioteknik; Biotechnology; Biokemi; Metabolism; Biochemistry; metal ion;

    Abstract : Metal affinity tags have been fused to proteins and demonstrated to function as tools in protein purification, protein stabilisation, quantitative metal analysis and metal tolerance of bacteria and plants. In biotechnological applications proteins frequently have to function in nonnatural environments with harsh conditions which impose requirements for high stability on the proteins. READ MORE

  7. 5. On the application and validation of multiplexed affinity assays

    Author : Tea Dodig-Crnković; Jochen M. Schwenk; Mun-Gwan Hong; Peter Nilsson; Norman Leigh Anderson; KTH; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Affinity proteomics; Antibody; Autoantibody; Multiplexed assays; Protein microarray; Plasma proteins; Suspension bead array; Bioteknologi; Biotechnology;

    Abstract : Proteins are essential macromolecules that carry out complex functions in human cells, tissues, and organs. They regulate a diverse set of biological processes and protect against pathogens. However, dysregulation or malformation of proteins can cause disease. READ MORE