Search for dissertations about: "affinity"

Showing result 1 - 5 of 1238 swedish dissertations containing the word affinity.

  1. 1. Protein engineering to explore and improve affinity ligands

    Author : Martin Linhult; KTH; []
    Keywords : binding; affinity; human serum albumin HSA ; albumin-binding domain ABD ; affinity chromatography; deamidation; protein A; stabilization; Z-domain; capacity; protein G; cleaning-in-place CIP ; protein engineering; C2 receptor;

    Abstract : In order to produce predictable and robust systems forprotein purification and detection, well characterized, small,folded domains descending from bacterial receptors have beenused. These bacterial receptors, staphylococcal protein A (SPA)and streptococcal protein G (SPG), possess high affinity to IgGand / or HSA. READ MORE

  2. 2. Protein microarrays for validation of affinity binders

    Author : Mårten Sundberg; Peter Nilsson; Jochen Schwenk; Anders Isaksson; Afshin Ahmadian; KTH; []
    Keywords : ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; Microarray; protein; antibody; antigen; affinity; validation;

    Abstract : Is specificity an important issue regarding affinity reagents? What about the validation of affinity reagents today, is it good enough? This depends on the application and the producer of the reagent. Validation should be the most important marketing argument that can be found. READ MORE

  3. 3. Insights into weak affinity antibody-antigen interactionsStudies using affinity chromatography and optical biosensor

    Author : Magnus Strandh; Institutionen för Immunteknologi; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Bioteknik; Biotechnology; immunosensor; biosensor; weak affinity chromatography; cross-reactivity; antibody-antigen interaction; weak affinity; biomolecular recognition; Immunology; serology; transplantation; Immunologi; serologi;

    Abstract : Molecular recognition and interaction is fundamental for the function of biological systems. The properties of the interacting biomolecules dictate the type of forces involved and the strength and dynamics of the interaction. Many interactions are very strong whereas others exhibit weak affinity. READ MORE

  4. 4. On the application and validation of multiplexed affinity assays

    Author : Tea Dodig-Crnković; Jochen M. Schwenk; Mun-Gwan Hong; Peter Nilsson; Norman Leigh Anderson; KTH; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; Affinity proteomics; Antibody; Autoantibody; Multiplexed assays; Protein microarray; Plasma proteins; Suspension bead array; Biotechnology; Bioteknologi;

    Abstract : Proteins are essential macromolecules that carry out complex functions in human cells, tissues, and organs. They regulate a diverse set of biological processes and protect against pathogens. However, dysregulation or malformation of proteins can cause disease. READ MORE

  5. 5. Exploring molecular interactions between polypeptide conjugates and protein targets : Manipulating affinity by chemical modifications

    Author : Aleksandra Balliu; Lars Baltzer; Oliver Seitz; Uppsala universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; polypeptide conjugates; protein targets; molecular recognition; affinity enhancement; conjugation;

    Abstract : In this thesis molecular interactions between polypeptide conjugates and protein targets were investigated. Polypeptides were derivatized with small organic molecules, peptides and oligonucleotides. New strategies were developed with the aim to increase affinities for proteins of biological interest. READ MORE