Search for dissertations about: "analytical ultracentrifugation"

Showing result 1 - 5 of 8 swedish dissertations containing the words analytical ultracentrifugation.

  2. 1. Asymmetrical Flow Field-Flow Fractionation in the Application to Biological Macromolecules

    Author : Cecilia Arfvidsson; Centrum för analys och syntes; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; Analytisk kemi; Bioteknik; Biotechnology; biotechnology; recombinant protein production; molecular weight; Escherichia coli; ribosomes; glutenin; asymmetrical flow field-flow fractionation; multiangle light scattering;

    Abstract : Asymmetrical flow field-flow fractionation (AsFlFFF) is especially useful in the application to biological macromolecules due to the absence of a stationary phase in the separation channel. As a result no upper exclusion limit occurs and low shear forces affect the sample components. READ MORE

  4. 2. Characterization of Amino Acid tRNA Ligases using the Analytical Ultracentrifuge

    Author : Lars Lundvik; Göteborgs universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; amino acid tRNA ligase; analytical ultracentrifugation;

    Abstract : ABSTRACT. Quaternary structures of amino acid tRNA ligases/synthetases (aaRS) in the native as well as in denatured forms were examined by molecular weight determinations (paper I-IV). Analytical ultracentrifugation, gel electrophoresis, gel chromatography were used in these investigations. READ MORE

  5. 3. Diagnostic methods for demonstration of intrathecal synthesis of immunoglobulines within the central nervous system

    Author : Sten Öhman; Linköpings universitet; []
    Keywords : MEDICINE; MEDICIN;

    Abstract : This thesis is based upon studies of quantitative and qualitative determinations of immunoglobulins A, G, and M (lgA, IgG, and IgM) in cerebrospinal fluid (CSF) and in serum. Inflammatory diseases, such as multiple sclerosis and infections (e.g. READ MORE

  6. 4. To reduce a ribonucleotide – Radical solutions in enzymology in form and function

    Author : Oskar Aurelius; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Ribonucleotide reductase; X-ray crystallography; Small-angle X-ray scattering; nucleotide metabolism; protein biophysics; metalloproteins; anaerobic enzymology;

    Abstract : It has been more than 50 years since the enzyme system ribonucleotide reductase (RNR), catalysing the reduction of ribonucleotides to deoxyribonucleotides, was first discovered. RNR was also the first time that radical chemistry was revealed in an enzyme. READ MORE

  7. 5. Protein-protein interactions in model systems : design, control of catalytic activity and biosensor applications

    Author : Johan Rydberg; Lars Baltzer; Joel Schneider; Linköpings universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Chemistry; Protein interactions; design; catalysis; biosensors; hybride materials; nanoparticles; Kemi; Chemistry; Kemi;

    Abstract : This thesis describes the design of polypeptides, unordered in the monomeric state but capable of folding into helix-loop-helix motifs and dimerise to form four-helix bundles. The goal of the design was to encode them with the capacity to form dimers highly selectively and the ability to carry out molecular functions in the folded state but not in the unordered state, and thus to establish a molecular link between recognition and function. READ MORE