Search for dissertations about: "and His-tag"

Showing result 1 - 5 of 7 swedish dissertations containing the words and His-tag.

2. 1. Ribosomal Stalk Protein L12 : Structure, Function and Application

   Author : Chandra Sekhar Mandava; Suparna Sanyal; Knud Nierhaus; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Ribosome; protein synthesis; L12 dimer; initiation; elongation; G factors; and His-tag; Molecular biology; Molekylärbiologi; Biology with specialization in Molecular Biology; Biologi med inriktning mot molekylärbiologi;

   Abstract : Ribosomal stalk proteins are known to play important role in protein synthesis. The ‘stalk’, an extended structure on the large subunit of the ribosome is composed mainly of two to three dimers of L12 and one L10 protein, which forms the base of the stalk. In E. READ MORE

4. 2. The Membrane-Spanning Domain of Complex I Investigated with Fusion Protein Techniques

   Author : Maria Trane; Biokemi och Strukturbiologi; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; NuoN; NuoM; NuoL; covalent bound heme; Bacillus subtilis; Escherichia coli; membrane protein; His-tag; Cytochrome c; NADH:quinone oxidureductase; fusion protein;

   Abstract : NADH:quinone oxidoreductase, or complex I, is a large, complex and poorly understood bioenergetic enzyme in the respiratory chain of living organisms. The enzyme has a conserved core structure, comprising fourteen protein subunits: Seven subunits protrude from the membrane and contain a flavin prosthetic group and eight iron-sulfur clusters that guide electrons from the oxidation of NADH towards the quinone binding site(s). READ MORE

5. 3. Glycoside hydrolases from extremophiles isolated in Iceland. alpha-L-Rhamnosidases and pectin degrading enzymes

   Author : Hakon Birgisson; Bioteknik; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; immobilization; Biotechnology; Bioteknik; crystallization; sequencing; cloning; screening; thermophile; psychrotroph; psychrophile; alpha-L-arabinofuranosidase; alpha-L-rhamnosidase; thermostable; cold active; polygalacturonase;

   Abstract : Extreme environments (high and low temperature, high and low pH, etc.) are inhabited by a vast and diverse flora of microorganisms, which are adapted to these environments (extremophiles). READ MORE

6. 4. Shining light on unknown function proteins

   Author : Riccardo Diamanti; Martin Högbom; Hans-Petter Hersleth; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; proteins; membrane proteins; metalloproteins; biochemistry; metabolism; biokemi; Biochemistry;

   Abstract : The study of protein structure and function is a fundamental step for understanding biological systems and for creating a solid ground for future drug development. Despite the development of novel ground-breaking biochemical and bioinformatical tools in the last decades, most proteins found in nature still have unknown assigned function. READ MORE

7. 5. Structural Studies of the Human Water Channel Protein Aquaporin 5

   Author : Anna Backmark; Chalmers tekniska högskola; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; aquaporin 5; aquaporins; X-ray crystallography; twinning; structure; trafficking;

   Abstract : Aquaporins are water channel proteins found in the membranes of all living cells. In humans, thirteen aquaporins have been identified to date. Many of them have been implicated in water imbalance disorders. Water transport through the aquaporins is regulated at several levels. READ MORE