Search for dissertations about: "anti transcriptase enzyme"

Showing result 1 - 5 of 12 swedish dissertations containing the words anti transcriptase enzyme.

2. 1. HIV-1 reverse transcriptase activity assays based on monoclonal antibodies to native enzyme : Applications on studies of antiviral substances and immunological variation of reverse transcriptase

   Author : Ann-Sofie Rytting; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; HIV-1; reverse transcriptase; monoclonal antibody; activity assay; subtype; RT inhibitor; Genetik; Clinical genetics; Klinisk genetik; Medical Genetics; medicinsk genetik;

   Abstract : Specific detection of HIV-1 reverse transcriptase (RT) activity in crude materials was enhanced using a capture assay for immunopurification and subsequent activity assay. The purification step was achieved by anti-HIV-1 RT monoclonal antibodies (Mabs) immobilized onto a solid carrier. READ MORE

4. 2. Towards a New Generation of Anti-HIV Drugs : Interaction Kinetic Analysis of Enzyme Inhibitors Using SPR-biosensors

   Author : Malin Elinder; U. Helena Danielson; Maurizio Botta; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; drug discovery; fragment; screening; reverse transcriptase; microbicides; interaction analysis; NNRTIs; Biochemistry; Biokemi; Biokemi; Biochemistry;

   Abstract : As of today, there are 25 drugs approved for the treatment of HIV and AIDS. Nevertheless, HIV continues to infect and kill millions of people every year. Despite intensive research efforts, both a vaccine and a cure remain elusive and the long term efficacy of existing drugs is limited by the development of resistant HIV strains. READ MORE

5. 3. Reverse Transcriptase Activity Assays for Retrovirus Quantitation and Characterization

   Author : Anders Malmsten; Ulf Gyllensten; Clas Källander; Lynn Morris; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Medicine; retrovirus; reverse transcriptase; enzyme activity assay; MuLV; HIV; RT purification; viral load; drug susceptibility; Medicin; Dermatology and venerology; clinical genetics; internal medicine; Dermatologi och venerologi; klinisk genetik; invärtesmedicin;

   Abstract : Reverse transcriptase (RT) is a crucial enzyme for retrovirus replication, and its presence in the virion is indispensable for infectivity. This thesis illustrates the use of RT activity assays as tools for quantitation and characterization of different retroviruses, particularly HIV. READ MORE

6. 4. HIV-1 reverse transcriptase as a target in the development of specific enzyme inhibitors

   Author : Hong Zhang; Karolinska Institutet; Karolinska Institutet; []
   Keywords : ;

   Abstract : Human immunodeficiency virus type I (HIV-I), which causes the acquired immunodeficiency syndrome (AIDS), begins its intracellular infection life cycle with reverse transcription of its plus-strand RNA genome into a double-stranded proviral DNA intermediate which is integrated into the host chromosome inducing a persistent infection. A virally encoded enzyme, reverse transcriptase (RT), carries out the reverse transcription process by performing all three enzymatic activities, i. READ MORE

7. 5. Reverse transcriptase assay for analysis of resistance to anti-HIV drugs and their mechanism of action

   Author : Xingwu Shao; Karolinska Institutet; Karolinska Institutet; []
   Keywords : HIV-1; RT; antiviral drugs; NRTls; NNRTls; drug resistance; viral load;

   Abstract : Reverse transcriptase (RT) is a viral enzyme and one of the main targets for drugs against human immunodeficiency virus (HIV). This work presents a set of sensitive non-radioactive RT assays were developed: standard RT inhibition assay (IC assay); RT binding inhibition assay; RTprotein detection assay and template-primer destruction assay or chain termination assay (CT assay). READ MORE