Search for dissertations about: "antibody purification"

Showing result 1 - 5 of 64 swedish dissertations containing the words antibody purification.

2. 1. Engineering of small IgG binding domains for antibody labelling and purification

   Author : Sara Kanje; Sophia Hober; Cecília Roque; KTH; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Antibody; labelling; purification; Protein G; Protein A; protein engineering; protein design; combinatorial selection; Bioteknologi; Biotechnology;

   Abstract : In protein engineering, rational design and selection from combinatorial libraries are methods used to develop proteins with new or improved features. A very important protein for the biological sciences is the antibody that is used as a detecting agent in numerous laboratory assays. READ MORE

4. 2. Systems enabling antibody-mediated proteomics research

   Author : Ronny Falk; Sophia Hober; Christer Wingren; KTH; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Antibody generation; dual expression; affinity purification; E. coli expression; Affibody molecules; polyclonal antibody; monospecific antibody; Immunobiology; Immunbiologi;

   Abstract : As many genome sequencing efforts today are completed, we are now provided with the genetic maps for several organisms, including man. With these maps at hand, the scientific focus is now shifting towards investigations of the functionality of proteins. READ MORE

5. 3. Affinity Two-Phase Partitioning of Liposomes and Membranes

   Author : Irene Barinaga-Rementeria Ramírez; Biokemi och Strukturbiologi; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; miniaturised two-phase system; levitated drop; biotin; avidin; antibody; immunoaffinity; purification; rat lung; rat liver; caveolae; membrane; Affinity two-phase partitioning; liposome; Metabolism; Biochemistry; Biokemi; metabolism;

   Abstract : Affinity two-phase partitioning based on an immunoaffinity sandwich approach for the rapid and selective purification of membranes is presented in this thesis. The method was developed by studying different parameters governing the affinity partitioning of model membranes. To this end, biotinylated liposomes were used. READ MORE

6. 4. Calcium-dependent Affinity Domains for the Purification of Antibodies and Antibody Fragments

   Author : Julia Scheffel; Sophia Hober; Alois Jungbauer; KTH; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Antibodies; Antibody fragments; Biomanufacturing; Protein purification; Protein engineering; Protein A; ZCa; Calcium; Mild elution; Bioteknologi; Biotechnology;

   Abstract : Antibodies are essential proteins in both our bodies and biotechnological research, and hold outstanding therapeutic value. The market for antibody-based therapeutics has grown exponentially during the last decades, owing to several advantages over small molecule drugs, such as fewer undesirable side effects associated with a higher target specificity. READ MORE

7. 5. Modeling Antibody Aggregation in Downstream Processing

   Author : Frida Ojala; Avdelningen för kemiteknik; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Antibody; Aggregation; Downstream processing; Size exclusion chromatography; Reversible reaction model; General rate model;

   Abstract : Antibodies are part of the immune system where their purpose is to recognize and neutralize foreign species such as viruses and bacteria, thus preventing and curing infections. The trend of using antibodies in the treatment of, for example, cancer is increasing. READ MORE