Search for dissertations about: "antibody validation"

Showing result 1 - 5 of 25 swedish dissertations containing the words antibody validation.

  1. 1. Protein microarrays for validation of affinity binders

    University dissertation from Stockholm : KTH Royal Institute of Technology

    Author : Mårten Sundberg; KTH.; [2011]
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Microarray; protein; antibody; antigen; affinity; validation;

    Abstract : Is specificity an important issue regarding affinity reagents? What about the validation of affinity reagents today, is it good enough? This depends on the application and the producer of the reagent. Validation should be the most important marketing argument that can be found. READ MORE

  2. 2. Towards subcellular localization of the human proteome using bioimaging

    University dissertation from Stockholm : KTH Royal Institute of Technology

    Author : Charlotte Stadler; KTH.; KTH.; [2012]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Antibody; antibody validation; automated image analysis; automated microscopy; cell line; confocal microscopy; fixation; green fluorescent protein GFP ; immunofluorescence IF ; organelle; protein expression; siRNA; subcellular localization;

    Abstract : Since the publication of the complete sequence of the human genome in 2003 there has been great interest in exploring the functions of the proteins encoded by the genes. To reveal the function of each and every protein, investigation of protein localization at the subcellular level has become a central focus in this research area, since the localization and function of a protein is closely related. READ MORE

  3. 3. Mass Spectrometry and Affinity Based Methods for Analysis of Proteins and Proteomes

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Mårten Sundberg; Uppsala universitet.; [2015]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Mass spectrometry; proteomics; microarray; protein; antibody; antigen; affinity; validation; Kemi med inriktning mot analytisk kemi; Chemistry with specialization in Analytical Chemistry;

    Abstract : Proteomics is a fast growing field and there has been a tremendous increase of knowledge the last two decades. Mass spectrometry is the most used method for analysis of complex protein samples. It can be used both in large scale discovery studies as well as in targeted quantitative studies. READ MORE

  4. 4. Epitope mapping of antibodies towards human protein targets

    University dissertation from Stockholm : KTH Royal Institute of Technology

    Author : Barbara Hjelm; KTH.; [2011]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; antibody; antibody validation; biomarker; epitope mapping; peptide array; proteomics; RBM3; staphylococcal surface display;

    Abstract : This thesis, based on five research papers, presents results from development and evaluation ofmethods for identifying the interaction site of antibodies on their antigens and the functional investigation of these in different assays. As antibodies have proven to be invaluable tools in diagnostics, therapy and basic research, the demand of characterizing these binding molecules has increased. READ MORE

  5. 5. Generation and characterization of antibodies for proteomics research

    University dissertation from Stockholm : KTH

    Author : Karin Larsson; KTH.; [2009]
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; antibody; antibody validation; immunohistochemistry; immunofluorescence; tissue microarray; Western blot; epitope mapping; antigen design; TECHNOLOGY Bioengineering; TEKNIKVETENSKAP Bioteknik;

    Abstract : Specific antibodies are invaluable tools for proteomics research. The availability of thoroughly validated antibodies will help to improve our understanding of protein expression, localization and function; fundamental processes and features of all living organisms. READ MORE