Search for dissertations about: "antisense oligonucleotides"

Showing result 11 - 15 of 53 swedish dissertations containing the words antisense oligonucleotides.

  2. 11. Membrane Interactions of Arginine-Rich Peptides for the Intracellular Delivery of Gene-Targeted Drugs

    Author : Per Thoren; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; cellular uptake; translocation; protein transduction; circular dichroism; drug delivery; liposomes; resonance energy transfer; PNA; antigene; antisense;

    Abstract : A major obstacle for the therapeutic use of e.g. oligonucleotides is their inherently poor cellular uptake. A recently discovered class of peptides, denoted cell-penetrating peptides, has been shown to traverse cell membranes, even when conjugated to oligonucleotides or even full-length proteins. READ MORE

  4. 12. Interactions of the Cell-Penetrating Peptide Penetratin with Model Membranes and Live Cells

    Author : Daniel Persson; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; antisense; antennapedia homeodomain; delivery vector; cell-penetrating peptide; protein transduction; uptake; membrane translocation; penetratin; confocal microscopy; vesicle;

    Abstract : The delivery of large, hydrophilic molecules, such as proteins and oligonucleotides, to the cytoplasm and nucleus of cells is problematic due to their generally low plasma membrane permeability. With the identification of a number of cell-penetrating peptides, capable of mediating non-endocytotic, intracellular delivery of macromolecular cargos, this obstacle now seems surmountable. READ MORE

  5. 13. Gene targeting and delivery of therapeutic oligonucleotides

    Author : Olof Gissberg; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : Research in gene therapeutic strategies involving oligonucleotides (ONs) constitutes a growing field with several clinical products already approved and many more under intense investigation. The recent advances have mainly involved the Antisense ON platform, with the aim of modulating gene expression on the RNA level. READ MORE

  6. 14. Bacterial gene expression inhibition with antisense peptide nucleic acids

    Author : Rikard Dryselius; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : Regulated gene expression is widely useful for gene function studies and as a means to control genes important in disease. There are many methods for regulating the expression of genes, and one approach is to use antisense oligonucleotides that bind complementary mRNAs to inhibit translation. READ MORE

  7. 15. Padlock probes : Circularizing oligonucleotides for localized detection of DNA sequence variants

    Author : Mats Nilsson; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; Genetik; Clinical genetics; Klinisk genetik; Medical Genetics; medicinsk genetik;

    Abstract : Present techniques for localized detection of DNA sequence variants have limitations due to problems with sensitivity, specificity, signal localization or ability to perform many analyses simultaneously.In this study a novel gene analytic reagent is presented - the padlock probe. READ MORE