Search for dissertations about: "antisense oligonucleotides"

Showing result 6 - 10 of 53 swedish dissertations containing the words antisense oligonucleotides.

2. 6. Computational prediction of antisense oligonucleotides and siRNAs

   Author : Alistair Chalk; Karolinska Institutet; Karolinska Institutet; []
   Keywords : Antisense oligonucleotides; siRNAs; Artificial neural networks; Support vector machines; prediction; specificity.;

   Abstract : Two popular gene knockdown methods, antisense oligonucleotides (AOs) and short interfering RNAs (siRNAs) are commonly used to selectively inhibit gene expression (gene knockdown). This is an extremely valuable tool for functional genomics, however the selection of effective molecules using either method is non trivial. READ MORE

4. 7. Radiolabelled Oligonucleotides for Evaluation of in vivo Hybridisation Utilising PET Methodology

   Author : Gábor Lendvai; Mats Bergström; Sergio Estrada; Barbro Eriksson; Raymond Josephsson; Bertrand Tavitian; Uppsala universitet; []
   Keywords : Molecular medicine; Gene expression; Antisense oligonucleotides; Positron emission tomography; In vivo hybridisation; In vivo biodistribution; Chromogranin-A; 68Ga; RT-PCR; Scavenger receptors; Molekylärmedicin;

   Abstract : Antisense oligonucleotides (ODN) may interfere in gene expression on the basis of hybridising to its complementary messenger RNA (mRNA) sequence in the cell thereby preventing the synthesis of the peptide. Therefore, these ODNs may be potential drugs to treat human diseases by “knocking down” the expression of responsible genes or correcting the maturation process of mRNA in the field called antisense therapy. READ MORE

5. 8. Targeting RNA by the Antisense Approach and a Close Look at RNA Cleavage Reaction

   Author : Jharna Barman; Lars Baltzer; Roger Strömberg; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Organic chemistry; mRNA targeting; antisense oligonucleotides; target affinity; RNase H; Michaelis-Menten kinetics; exo-nuclease stability; NMR; pH titration; alkaline hydrolysis; Organisk kemi;

   Abstract : This thesis summarizes the results of studies on two aspects of nucleic acids. Chemically modified antisense oligonucleotides (AONs) have been evaluated with regards to their suitability for mRNA targeting in an antisense approach (Paper I – III). READ MORE

6. 9. Chemically Modified Oligonucleotides: Synthesis, Physicochemical and Biochemical Properties of their Duplexes with DNA and RNA

   Author : Pushpangadan Indira Pradeepkumar; Jyoti Chattopadhyaya; Jesper Wengel; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Bioorganic chemistry; Antisense oligonucleotides; Dipyridophenazine; Oxetane; RNase H; Gene down-regulation; DNase 1; Charge transport; Bioorganisk kemi; Bioorganic chemistry; Bioorganisk kemi;

   Abstract : This thesis is based on 9 papers dealing with the synthesis, physicochemical and biochemical properties of two types of chemically modified oligonucleotides which have the potential to down-regulate gene expression: (i) The first set is comprised of antisense oligonucleotides (AONs) conjugated with different chromophores of varying size, charge and π-electron density. Conjugation of the chromophores at the 3'- or 5'-end enhanced the target RNA binding affinity and RNase H recruitment capabilities compared to the native counterpart without changing the global helical conformation of their AON/RNA hybrid duplexes. READ MORE

7. 10. Conformationally Constrained Oligonucleotides for RNA Targeting

   Author : Qing Li; Jyoti Chattopadhyaya; Li-He Zhang; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; conformationally constrained nucleoside; antisense oligonucleotide; RNA affinity; nuclease stability; RNase H; RNA degradation; Chemistry with specialization in Bioorganic Chemistry; Kemi med inriktning mot bioorganisk kemi;

   Abstract : A short oligonucleotide sequence as in a single-stranded antisense oligo nucleotides (AON) or in double-stranded small interfering RNAs (siRNA) can modulate the gene expression by targeting against the cellular mRNA, which can be potentially exploited for therapeutic purposes in the treatment of different diseases. In order to improve the efficacy of oligonucleotide-based drugs, the problem of target affinity, nuclease stability and delivery needs to be addressed. READ MORE