Search for dissertations about: "assay design"

Showing result 6 - 10 of 151 swedish dissertations containing the words assay design.

  2. 6. Cyclotides : Tuning Parameters Toward Their Use in Drug Design

    Author : Mariamawit Yonathan Yeshak; Ulf Göransson; Lars Bohlin; Kaleab Asres; Robert Kiss; Uppsala universitet; []
    Keywords : cyclotide; Viola abyssinica; Viola odorata; Extraction; Violaceae; vaby; cytotoxic; genotoxic; cell penetrating; microautoradiography; LC-MS; comet assay; cycloviolacin O2; Farmakognosi; Pharmacognosy;

    Abstract : Cyclotides are plant proteins with a unique topology, defined as the cyclic cystine knot motif. The motif endows cyclotides with exceptional chemical and biological stability. They also exhibit a wide range of biological activities including insecticidal, cytotoxic, anti-HIV and antimicrobial effects. READ MORE

  4. 7. Improved methodologies for molecular detection and quantification of viruses in food

    Author : Sofia Persson; Patrik Ellström; Magnus Simonsson; Wim van der Poel; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; foodborne virus; digital PCR; real-time PCR; validation; assay design; food safety; Medicinsk vetenskap; Medical Science;

    Abstract : Foodborne viruses such as norovirus, hepatitis A virus and hepatitis E virus cause a high burden of disease worldwide. Reverse transcription (RT) quantitative real-time PCR (qPCR) is the current standard method for monitoring viral contamination in the food chain. READ MORE

  5. 8. Advancing DNA-based proximity methods

    Author : Mattias Leino; Ola Söderberg; John Löfblom; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; in situ; proximity dependent intiation of hybridization chain reaction; hybridization chain reaction; Oligonucleotide design; proximity ligation assay; protein interaction; microscopy; purification; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

    Abstract : Cellular functions are governed by intricate chains of interactions between proteins. In order to properly understand cellular biology one must look into, not only protein function, but also its interacting network. READ MORE

  6. 9. Design and Synthesis of Galectin Inhibitors

    Author : Pernilla Sörme; Barbro Kahl Knutson; U Wellmar; B-G Magnusson; Hakon Leffler; Ulf Nilsson; Yuan C. Lee; Reiko T. Lee; Centrum för analys och syntes; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Parallel Synthesis; Inhibitors; Biomedicinska vetenskaper allmänt ; General biomedical sciences; Carbohydrates; Galectins;

    Abstract : Galectins, a lectin family, have shown binding affinities towards b-galactosides. Galectins have been proposed to be involved in a wide range of functions like for example, cell growth, adhesion, migration, chemo taxis and apoptosis. They have also been associated with various cancer types. READ MORE

  7. 10. Design of solid dosage forms for mucosal vaccination - Investigations on the influence of excipients on product performance

    Author : Annika Borde; Chalmers tekniska högskola; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; hydrophilic matrix tablets; osmotic pressure; protein release; freeze-drying; Vibrio cholerae; mucoadhesion; Bradford Assay; sublingual vaccine tablet; mucosal vaccination;

    Abstract : Most vaccines today are liquid formulations for parental administration. However, there are several drawbacks connected to these vaccines. Since injectable vaccines only induce systemic antibody responses, they are not effective against the various pathogens that affect mucosal surfaces with poor permeability for serum-derived antibodies, e.g. READ MORE