Search for dissertations about: "assay yeast"

Showing result 1 - 5 of 46 swedish dissertations containing the words assay yeast.

  2. 1. Engineering Yeast for Functional Analysis of Human Cu Transport Proteins

    Author : Kumaravel Ponnandai Schanmugavel; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Cu transport; yeast model; Atox1; genetic engineering; Wilson disease protein; ATP7B; complementation assay; yeast growth; metal-binding domain; mechanisms.;

    Abstract : ABSTRACT       Copper (Cu) is an important trace element that plays a vital role in several biological processes. Many proteins that are involved in various biochemical pathways require Cu as a cofactor. READ MORE

  4. 2. CRISPRi/a for investigating yeast tolerance to inhibitors in lignocellulosic hydrolysates

    Author : Ibai Lenitz Etxaburu; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Lignocellulosic hydrolysates; ChIP-exo; Screening; Biosensor; Formic acid; Competitive growth assay; Yeast; Tolerance; CRISPRi a; Acetic acid;

    Abstract : Saccharomyces cerevisiae has immense potential as a cell factory in various biotechnological processes where biomass from agricultural industry residues is used as feedstock. Nonetheless, the inhibitors released during the pretreatment of the biomass makes lignocellulosic hydrolysates a challenging substrate for microorganisms. READ MORE

  5. 3. Structure and Function of Aqua(glycero)porins - A Path to Drug Design

    Author : Gerhard Fischer; Göteborgs universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; structural biology; drug screening; Malaria; membrane proteins; yeast;

    Abstract : Membrane proteins are a major class of the drug targets. Rational design of drugs requires knowledge about the structure and function of these proteins. Working with membrane proteins, however, is experimentally challenging as the required technology is still emerging. READ MORE

  6. 4. Regulation and mechanism of mating-type switching in Kluyveromyces lactis

    Author : Naghmeh Rajaei; Stefan Åström; Kenneth Wolfe; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; yeast; mating-type switching; DSB; gene conversion; transposable elements; RAS cAMP pathway; nutrient limitation; molekylär biovetenskap; Molecular Bioscience;

    Abstract : Transposable elements (TEs) have had immense impact on the structure, function and evolution of eukaryotic genomes. The work in this thesis identified Kat1, a novel domesticated DNA transposase of the hAT family in the yeast Kluyveromyces lactis. Kat1 triggers a genome rearrangement that results in a switch of mating type from MATa to MATα. READ MORE

  7. 5. PCR-based detection of microorganisms in complex biological samples

    Author : Pär-G Lantz; Teknisk mikrobiologi; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Density gradient centrifugation; DNA extraction; Aqueous two-phase systems; Meat; Faeces; Cheese; Yeast; Helicobacter pylori; Yersinia enterocolitica; Listeria monocytogenes; PCR; Inhibition; Sample preparation; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Abstract : The detection of microorganisms by PCR can be divided into four steps: (1) sample collection, (2) sample preparation, (3) DNA amplification and (4) detection of PCR products. The major problem in developing PCR-based detection methods is the sample preparation step. READ MORE