Search for dissertations about: "binding modes"

Showing result 6 - 10 of 89 swedish dissertations containing the words binding modes.

  2. 6. NMR structral studies on ruthenium complexes binding to DNA

    Author : Lisha Wu; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NMR; DNA binding; Ru-complex dimer; CD; fluorescence; molecular dynamics simulation;

    Abstract : The Ru-complex Λ,Λ-[bidppz(bipy)4Ru2]4+ (Λ,Λ-B) exhibits multiple modes of interactions with the palindromic DNA dodecamer [d(CGCGAATTCGCG)]2, however excluding any type of intercalation as shown by both optical spectroscopy and NMR. NMR observation of Λ,Λ-B titration into a DNA solution results in both a transient binding, with observation of fast exchange for protons located near the ends of the DNA duplex, and a more stable interaction in the DNA minor groove indicated by a slow exchange mechanism and binding sites for G2, G4 and A5 of the same DNA strand. READ MORE

  4. 7. A biophysical study of nucleic acid interactions with analogues and drugs

    Author : Ulrica Sehlstedt; Donald M Crothers; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Nucleic acids; binding modes; polarised light spectroscopy; linear dichroism; NMR; triple helix; base-pair breathing dynamics; DAPI; porphyrins; quinoxaline derivatives; Biophysics; Biofysik; Biophysics; biofysik;

    Abstract : work presented in this thesis concerns studies on the physicochemical nature of interactions between nucleic acids and small ligands. The outcome of such studies can yield insights at a molecular level into the physiological mechanisms of action of biologically active nucleic-acid binding molecules. READ MORE

  5. 8. Amphiphilic Peptide Interactions with Complex Biological Membranes : Effect of peptide properties on antimicrobial and anti-inflammatory effects

    Author : Shalini Singh; Martin Malmsten; Per Hansson; Debora Berti; Uppsala universitet; []
    Keywords : LPS; Antimicrobial; Peptide; Inflammation; Infections; Liposome; Binding; PEG; Farmaceutisk fysikalisk kemi; Pharmaceutical Physical Chemistry;

    Abstract : With increasing problem of resistance development in bacteria against conventional antibiotics, as well as problems associated with diseases either triggered or enhanced by infection, there is an urgent need to identify new types of effective therapeutics for the treatment of infectious diseases and its consequences. Antimicrobial and anti-inflammatory peptides have attracted considerable interest as potential new antibiotics in this context. READ MORE

  6. 9. DNA Electrophoresis in Polymer Solutions. Separation Mechanisms studied by Fluorescence and Linear Dichroism Spectroscopy

    Author : Christina Carlsson; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; mutation detection; orientation; YOYO; DNA electrophoresis; polarized light spectroscopy; optical probing; polymer solutions; fluorescence microscopy; PNA; binding modes;

    Abstract : Capillary electrophoresis of DNA in polymer solutions is a promising technique for separation of DNA molecules according to size. The primary aim of the work presented in this thesis was to get a better understanding of the electrophoretic separation mechanism in these media by studying the DNA migration behavior during electrophoresis in both entangled and non-entangled polymer solutions using linear dichroism spectroscopy and fluorescence video microscopy. READ MORE

  7. 10. DNA Electrophoresis: Optical Probing and Microscopic Imaging to Understand Separation Mechanisms

    Author : Anette Larsson; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; optical probing; YOYO; polarized light spectroscopy; DNA electrophoresis; DAPI; binding modes; helix alignment; fluorescence microscopy;

    Abstract : The aim of this work was to combine spectroscopic, mobility, and fluorescence microscopy studies to elucidate the mechanisms behind DNA electrophoresis, a method to separate DNA molecules according to size. The microscopy studies require staining of the DNA with a fluorescent dye. READ MORE