Search for dissertations about: "binding specificity"

Showing result 1 - 5 of 366 swedish dissertations containing the words binding specificity.

2. 1. Novel binding specificities engineered into the scaffold of a carbohydrate binding module

   Author : Lavinia Cicortas Gunnarsson; Institutionen för immunteknologi; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Bioteknik; Biotechnology; carbohydrate binding module; binding specificity; selection; combinatorial library; scaffold; protein engineering;

   Abstract : The growing field of biotechnology is in constant need of proteins that can function as recognition tools for separational, analytical and therapeutic purposes. Different molecular engineering approaches are applied on natural proteins in order to create variants with desired properties. READ MORE

4. 2. Surface Properties of Antibodies and their Complexes with Antigens Studied by LLPC

   Author : Christer Wingren; Institutionen för immunteknologi; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; ligand-binding.; surface properties; isomerism; conformational changes; antibody; specificity; two-phase partitioning; antigen-binding sites; Biochemistry; Metabolism; Biokemi; metabolism;

   Abstract : The main objective of this thesis was to gain further insight into the relationship between the exposed surfaces and the functional properties of antibodies and their complexes with antigens in solution. To study this relationship, a new technique, liquid-liquid partition chromatography (LLPC) in aqueous two-phase systems, was developed further with respect to sensitivity, selectivity and reproducibility. READ MORE

5. 3. Structural Studies of Binding Proteins: Investigations of Flexibility, Specificity and Stability

   Author : Ulrika Magnusson; Sherry L. Mowbray; Anthony J. Wikinson; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Molecular biology; Periplasmic binding protein; X-ray crystallography; conformational change; acidophile; protein specificity; protein structure; Molekylärbiologi; Molecular biology; Molekylärbiologi;

   Abstract : Binding proteins are present both in gram-negative and gram-positive bacteria. They are the recognition components of the ABC transport systems that transport different nutrients into the cell, and are in some cases also involved in chemotaxis. READ MORE

6. 4. PARN – The Tail End : Function and mechanisms of specificity and processivity

   Author : Mikael Nissbeck; Anders Virtanen; Renée Schroeder; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Poly A specific ribonuclease; Adenosine; Specificity; Processivty; Telomere biology disorders; Molekylär cellbiologi; Molecular Cellbiology;

   Abstract : Poly(A)-specific ribonuclease (PARN) is an exoribonuclease that is processive, poly(A) specific and cap-binding. PARN deadenylates the poly(A) tails present on a subset of mRNAs and non-coding RNAs, including among others certain snoRNAs, miRNAs and precursor rRNAs. READ MORE

7. 5. Evolution and Binding Mechanisms of Intrinsically Disordered Proteins

   Author : Elin Karlsson; Per Jemth; Kaare Teilum; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Intrinsically disordered proteins; Protein evolution; Binding kinetics; Binding mechanisms; Biophysical characterisation; Medicinsk biokemi; Medical Biochemistry;

   Abstract : Intrinsically disordered proteins (IDPs) make up a considerable fraction of the proteome in eukaryotic organisms. These proteins often act as hubs in interaction networks, harbouring multiple interaction with other proteins, and thus evolution has to walk a tightrope to accommodate new interactions while maintaining the previously established interactions. READ MORE