Search for dissertations about: "bispecific"

Showing result 1 - 5 of 16 swedish dissertations containing the word bispecific.

  1. 1. An albumin-binding domain as a scaffold for bispecific affinity proteins

    Author : Johan Nilvebrant; Sophia Hober; Andrew Bradbury; KTH; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; NATURVETENSKAP; TEKNIK OCH TEKNOLOGIER; NATURAL SCIENCES; ENGINEERING AND TECHNOLOGY; albumin-binding domain; bispecific; albumin; affinity protein; phage display; staphylococcal display; orthogonal affinity purification; TNF-alpha; ErbB2; ErbB3; SRA - Molekylär biovetenskap; SRA - Molecular Bioscience;

    Abstract : Protein engineering and in vitro selection systems are powerful methods to generate binding proteins. In nature, antibodies are the primary affinity proteins and their usefulness has led to a widespread use both in basic and applied research. READ MORE

  2. 2. Engineering strategies for ABD-derived affinity proteins for therapeutic and diagnostic applications

    Author : Mikael Åstrand; Sophia Hober; John Löfblom; Larry Gold; KTH; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ABD; ADAPT; Protein G; ERBB2; ERBB3; Directed evolution; phage display; staphylococcal display; bispecific; Biotechnology; Bioteknologi;

    Abstract : Small stable protein domains are attractive scaffolds for engineering affinity proteins due to their high tolerance to mutagenesis without loosing structural integrity. The albuminbinding domain is a 5 kDa three-helix bundle derived from the bacterial receptor Protein G with low-nanomolar affinity to albumin. READ MORE

  3. 3. EGFR- and HER2-Binding Affibody Molecules : Cellular studies of monomeric, dimeric and bispecific ligands

    Author : Lina Ekerljung; Lars Gedda; Johan Lennartsson; Jörgen Carlsson; Birgitta Sundström; Uppsala universitet; []
    Keywords : EGFR; HER2; Affibody molecule; cell signaling; receptor dimerization; Biomedical Radiation Science; Biomedicinsk strålningsvetenskap;

    Abstract : Abnormal expression and signaling of the ErbB receptors is associated with the development and progression of several forms of cancer. In this thesis, new ErbB-targeting affibody molecules are evaluated regarding their cellular effects in vitro. READ MORE

  4. 4. Preclinical PET imaging and therapy of Alzheimer's disease

    Author : Silvio R. Meier; Stina Syvänen; Dag Sehlin; Greta Hultqvist; Matthias Brendel; Uppsala universitet; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Alzheimer s disease; amyloid-beta; positron emission tomography PET ; bispecific antibody; BACE-1; TREM2; GFAP; Molekylär medicin; Molecular Medicine; Neuroscience; Neurovetenskap;

    Abstract : The main histopathological hallmarks of Alzheimer’s disease are extracellular amyloid-beta (Aβ) plaques and intracellular neurofibrillary tangles, containing tau protein. Because of misfolded and aggregated proteins, activated microglia and astroglia react with a neuroinflammatory response, which may contribute to disease progression and severity. READ MORE

  5. 5. Preclinical PET imaging of Alzheimer's disease progression

    Author : Xiaotian T. Fang; Stina Syvänen; Dag Sehlin; Lars Lannfelt; Merja Haaparanta-Solin; Uppsala universitet; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICAL AND HEALTH SCIENCES; Alzheimer s disease; transgenic mice; PET; antibody-based tracer; mGluR5; ABP688; di-scFv; amyloid-β;

    Abstract : Amyloid PET imaging with [11C]PIB enabled detection of Aβ for the first time in vivo. However, [11C]PIB is a small molecule that binds only the insoluble Aβ plaque. Rather, the soluble Aβ aggregates are considered the cause of Alzheimer’s disease (AD). READ MORE