Search for dissertations about: "calbindin D9k"

Showing result 1 - 5 of 7 swedish dissertations containing the words calbindin D9k.

  1. 1. Shaking the EF-Hand. Dynamics and Ion-Binding to Calmodulin and Calbindin D9k

    University dissertation from Anders Malmendal, Physical Chemistry 2, Lund University, P.O.Box 124, S-221 00 Lund, Sweden

    Author : Anders Malmendal; Lund University.; Lunds universitet.; [1998]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; magnesium; line shape; ion binding; EF-hand; cooperativity; conformational exchange; chemical shift; calmodulin; calcium; calbindin D9k; allostery; backbone dynamics; NMR; relaxation; Physical chemistry; Fysikalisk kemi;

    Abstract : The work described in this thesis represents a biophysical approach, mainly using nuclear magnetic resonance (NMR) spectroscopy, to investigate the impact of magnesium ions on the EF-hand calcium-binding proteins calmodulin and calbindin D9k, as well as the dynamics of these proteins at low calcium levels. The x-ray structure of magnesium-saturated calbindin D9k shows a magnesium ion in only one of the two ion binding sites. READ MORE

  2. 2. The effect of sequence truncation and amino-acid substitutions on the biophysical properties of protein S and calbindin D9k

    University dissertation from Karin Julenius, Physical Chemistry 2, Lund University, P.O.B. 124, S-221 00 Lund, Sweden

    Author : Karin Julenius; Lund University.; Lunds universitet.; [1999]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; surface plasmon resonance; fluorescence spectroscopy; circular dichroism spectroscopy; NMR spectroscopy; hydrophobic effect; protein stability; dimerization; cooperativity; calcium binding; EGF-module; EF-hand; calbindin D9k; protein S; synthet; Physical chemistry; Fysikalisk kemi;

    Abstract : Protein S is a modular protein and a cofactor in the protein C anticoagulant system. It consists of a Gla-module, a thrombin-sensitive loop, four EGF-modules and a SHBG-like module. READ MORE

  3. 3. Protein Interactions: Electrostatics and Ligand Binding

    University dissertation from Department of Biophysical Chemistry, Lund University

    Author : Ingemar André; Lund University.; Lunds universitet.; [2005]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Molecular biophysics; Molekylär biofysik; secondary structure formation; protein GB1; pKa values; pH stability; NMR; electrostatic interactions; M proteins; calbindin D9k; calcium binding; cooperativity; calmodulin; calbindin D28k;

    Abstract : This thesis deals with Ca2+ binding to proteins, electrostatic interactions in and between proteins as well as inter- and intramolecular interactions. A computer program was developed to determine Ca2+ binding constants from experimental titration data of proteins. READ MORE

  4. 4. Protein Complexity via Non-Native States: Binding, Stability, and Structural Studies of Calbindin D9k and HAMLET (Human alpha-lactalbumin Made LEthal to Tumor cells)

    University dissertation from Jonas Fast, Biophysical Chemistry Lund University

    Author : Jonas Fast; Lund University.; Lunds universitet.; [2003]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; apoptosis; oleic acid; HAMLET; kinetic trap alpha-lactalbumin; calbindin; calcium; Metabolism; Biokemi; metabolism;

    Abstract : Using optical spectroscopy, nuclear magnetic resonance (NMR), and differential scanning Calorimetry (DSC), I have studied two different calcium binding proteins that can form kinetically trapped altered states. Calcium is very important in numerous biological processes such as blood coagulation, signal transduction, muscle contraction and bone formation. READ MORE

  5. 5. Structural studies of metal-binding proteins revealing dynamic behaviour

    University dissertation from Molecular Biophysics, Lund University

    Author : Maria Håkansson; Lund University.; Lunds universitet.; [2001]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Kemi; Chemistry; Molekylär biofysik; Molecular biophysics; zinc; magnesium; superantigen; calbindin D9k; X-ray crystallography; staphylococcal enterotoxin; structure; Chemical technology and engineering; Kemiteknik och kemisk teknologi;

    Abstract : The Mg2+ and Mn2+ structures of calbindin D9k have been determined and they reveal a conformational change compared to the structures of apo and (Ca2+)2-calbindin D9k. These findings are unique, since normally calcium and not magnesium is known to induce conformational changes of EF-hand proteins. READ MORE