Search for dissertations about: "carbohydrate binding domains"

Showing result 1 - 5 of 18 swedish dissertations containing the words carbohydrate binding domains.

  2. 1. Production and investigation of highly thermophilic multi-domain carbohydrate-active enzymes

    Author : Daniel Krska; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; protein structure; xylanase; carbohydrate esterase; multidomain enzymes; carbohydrate-active enzymes; thermostable enzymes; plant biomass degradation; Caldicellulosiruptor;

    Abstract : With the looming threat of climate change caused largely by an excess of carbon dioxide in the atmosphere, recent scientific efforts have focused on the substitution of fossil fuels and other polluting compounds with more environmentally conscious choices. To this end, the investigation of biomass as both a renewable source of energy and as a chemical basis to produce high-value products is being extensively investigated. READ MORE

  4. 2. RECOMBINANT PEROXIDASES AND XYLANASES: I. Cloning and production of a peroxidase from horseradish II. Characterisation of functional domains of thermostable xylanases from Rhodothermus marinus

    Author : Eva Bartonek Roxå; Bioteknik; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; amorphous cellulose; xylan; CBD; carbohydrate binding domains; insect cells; Baculovirus; catalytically active; recombinant horseradish peroxidase; HRP-n; PASC; beta-glucan; Biotechnology; Bioteknik;

    Abstract : Part I: Plant peroxidases are useful in many areas of application e.g. as tracer enzymes for clinical applications and in biosensors as well as for industrial purposes like in waste water treatment and bleaching processes. A gene encoding a new, neutral horseradish peroxidase isoenzyme, HRP-n, was isolated. READ MORE

  5. 3. On the engineering of proteins: methods and applications for carbohydrate-active enzymes

    Author : Fredrika Gullfot; Harry Brumer; Antoni Planas; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; enzyme engineering; rational design; directed evolution; DNA shuffling; glycosynthase; xyloglucan; xyloglucan endo-transglycosylase; retaining glycoside hydrolase; xyloglucanase; carbohydrate binding module; polysaccharide synthesis; Bioengineering; Bioteknik;

    Abstract : This thesis presents the application of different protein engineering methods on enzymes and non-catalytic proteins that act upon xyloglucans. Xyloglucans are polysaccharides found as storage polymers in seeds and tubers, and as cross-linking glucans in the cell wall of plants. READ MORE

  6. 4. A beta-phosphoglucomutase in carbohydrate metabolism of Lactococcus lactis

    Author : Ny Qian; Teknisk mikrobiologi; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; mykologi; virologi; bakteriologi; Mikrobiologi; mycology; gene regulation; beta-phosphoglucomutase; maltose metabolism; lactate; metabolic engineering; alpha-phosphoglucomutase; Lactococcus lactis; Microbiology; virology; bacteriology; Biochemistry; Metabolism; Biokemi;

    Abstract : Phosphoglucomutase (PGM) catalyzes the reversible conversion of glucose 1-phosphate to glucose 6-phosphate. Two distinct forms of PGM have been purifed and charactierized in Lactococcus lactis growing on maltose: one specific for the alpha-isomer, and the other specific for the beta-isomer of glucose 1-phosphate. READ MORE

  7. 5. Plant UDP-glucose Pyrophosphorylase : Function and Regulation

    Author : Meng Meng; Leszek Kleczkowski; Javier Pozueta-Romero; Umeå universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; UDP-glucose pyrophosphorylase; carbohydrate metabolism; in vivo regulation; T-DNA knockout; heterologous expression; isozyme; mutagenesis; kinetic property; oligomerization; Plant physiology; Växtfysiologi;

    Abstract : UDP-glucose pyrophosphorylase (UGPase) is an important enzyme of carbohydrate metabolism in all living organisms. The main aim of this thesis was to investigate the function and regulation of plant UGP genes as well as the UGPase proteins. READ MORE