Search for dissertations about: "carbohydrate binding modules"

Showing result 1 - 5 of 7 swedish dissertations containing the words carbohydrate binding modules.

2. 1. Functional studies and engineering of family 1 carbohydrate-binding modules

   Author : Janne Lehtiö; KTH; []
   Keywords : Cellulose-binding; carbohydrate-binding modules; phage display; bacterial surface display; combinatorial protein library; protein engineering; Trichoderma reesei; Staphylococcus carnosus;

   Abstract : The family 1 cellulose-binding modules (CBM1) form a groupof small, stable carbohydrate-binding proteins. These modulesare essential for fungal cellulosedegradation. This thesisdescribes both functional studies of the CBM1s as well asprotein engineering of the modules for several objectives. READ MORE

4. 2. Heterologous expression, characterization and applications of carbohydrate active enzymes and binding modules

   Author : Åsa Kallas; Tuula T. Teeri; Edward A. Bayer; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES; Populus; xyloglucan endotransglycosylase; carbohydrate binding modules; endo- 1; 4 -b-xylanase; Escherichia coli; Pichia pastoris; N-glycosylation; fiber analysis; Bioengineering; Bioteknik; Wood fibre and forest products; Träfiber- och virkeslära;

   Abstract : Wood and wood products are of great economical and environmental importance, both in Sweden and globally. Biotechnology can be used both for achieving raw material of improved quality and for industrial processes such as biobleaching. READ MORE

5. 3. Engineering protein-carbohydrate interactions - lessons from natural and evolved carbohydrate binding modules

   Author : Laura von Schantz; Institutionen för immunteknologi; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; carbohydrate binding modules protein engineering affinity affinity maturation phage display specificity crystallography cellulose derivatives;

   Abstract : Protein-carbohydrate interactions are of central role in all living organism. In the studies presented in this thesis several strategies for engineering such interactions have been investigated. In the first study the binding affinity of a carbohydrate binding module (CBM) was improved a 100-fold through affinity maturation. READ MORE

6. 4. On the engineering of proteins: methods and applications for carbohydrate-active enzymes

   Author : Fredrika Gullfot; Harry Brumer; Antoni Planas; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; enzyme engineering; rational design; directed evolution; DNA shuffling; glycosynthase; xyloglucan; xyloglucan endo-transglycosylase; retaining glycoside hydrolase; xyloglucanase; carbohydrate binding module; polysaccharide synthesis; Bioengineering; Bioteknik;

   Abstract : This thesis presents the application of different protein engineering methods on enzymes and non-catalytic proteins that act upon xyloglucans. Xyloglucans are polysaccharides found as storage polymers in seeds and tubers, and as cross-linking glucans in the cell wall of plants. READ MORE

7. 5. Mannan-hydrolysis by hemicellulases: enzyme-polysaccharide interaction of a modular beta-mannanase

   Author : Per Hägglund; Biokemi och Strukturbiologi; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Metabolism; Biochemistry; hemicellulase; beta-mannanase; carbohydrate-binding modules; Biokemi; metabolism;

   Abstract : The enzymatic degradation of plant polysaccharides is a process of fundamental importance in nature which involves a wide range of enzymes. In this work, the structure and function of hemicellulose-degrading enzymes was investigated. The focus was on a beta-mannanase (TrMan5A) produced by the filamentous fungus Trichoderma reesei. READ MORE