Search for dissertations about: "cassette mutagenesis"

Found 2 swedish dissertations containing the words cassette mutagenesis.

  2. 1. Protein Engineering on Azurin. Expression Mutagenesis and Characterisation of Copper Site Mutants

    Author : B Göran Karlsson; Chalmers tekniska högskola; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; EPR spectroscopy; cassette mutagenesis; optical spectroscopy; amino acid exchange; Escherichia coli; electron transfer; reduction; azurin;

    Abstract : Azurin belongs to a family of small blue copper proteins or cupredoxins which participate in electron transfer processes in plants and bacteria. The type 1 copper site in these proteins is characterised by an intense blue colour, a narrow hyperfine coupling in the EPR signal and, generally, a high reduction potential. READ MORE

  4. 2. Mechanisms and DNA Specificity in Site-specific Recombination of Integron Cassettes

    Author : Carolina Johansson; Lars Sundström; Dan Andersson; Didier Mazel; Uppsala universitet; []
    Keywords : Microbiology; lateral gene transfer; site-specific recombination; tyrosine recombinase; integron; single-stranded; DNA hairpin; Mikrobiologi; farmaceutisk mikrobiologi; Pharmaceutical Microbiology;

    Abstract : Bacterial resistance to antibiotics has become a serious problem. This is due to the remarkable ability of bacteria to respond and rapidly adapt to environmental changes. Integrons are elements with the capacity for gene capture by an integron-encoded site-specific recombinase called IntI. READ MORE