Search for dissertations about: "cell clarification"

Showing result 1 - 5 of 11 swedish dissertations containing the words cell clarification.

  2. 1. From single-molecule sensing to extracellular vesicles in glioma cells under stress

    Author : Virginia Claudio; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proteome; single molecule sensing; nanoplasmonics; surface plasmon resonance; exosomes; glioblastoma; extracellular vesicles; cellular stress; lipid composition;

    Abstract : This thesis describes the work I conducted in two different areas, namely biomolecular sensing and quantitative cell biology. The work in the former area was focused on the optimization of plasmonic metamaterials for sensing applications, and in the latter on the characterization of extracellular vesicles released by glioblastoma cells under stress conditions. READ MORE

  4. 2. Magnetic bead-based isolation of biological therapeutic modalities

    Author : Nils A. Brechmann; Véronique Chotteau; Nigel Titchener-Hooker; KTH; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Magnetic bead separation; Magnetic beads; Protein A; Protein G; Negative cell isolation; Monoclonal antibody; induced pluripotent stem cells; human mesenchymal stem cells; Allogenic cell therapy; Biomanufacturing; cell clarification; Biotechnology; Bioteknologi;

    Abstract : Biopharmaceutical modalities, such as monoclonal antibodies or the less established cell therapies, are nowadays very important for the treatment of severe or incurable diseases. The manufacturing of such modalities is complex and costly, including the downstream processing, which is highly essential to ensure the safety and quality of the product. READ MORE

  5. 3. Purification Processes for Complex Biomacromolecules

    Author : Hans Blom; Helena Grennberg; Mats Lundgren; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Chromatography; enzymes; mucins; fractionation; plasmid DNA; analysis; clarification; process design; Chemistry with specialization in Surface Biotechnology; Kemi med inriktning mot ytbioteknik;

    Abstract : This thesis details various techniques and considerations for the purification of complex biomacromolecules. Initially an α-mannosidase from babaco fruit was purified using anion exchange-, lectin affinity- and size exclusion chromatography. READ MORE

  6. 4. Nucleic Acids: Innovative Methods for Recovery, Clarification and Purification

    Author : Tiago Matos; Tillämpad biokemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES;

    Abstract : The importance of nucleic acids in pure form for preparative and analytical perspectives, have increased constantly, demanding the development of new and more efficient methods for their recovery and isolation. This thesis describes a series of different innovative methods for recovery and purification of these biomolecules. READ MORE

  7. 5. Streptomyces sporulation - Genes and regulators involved in bacterial cell differentiation

    Author : Jessica Larsson; Biologiska institutionen; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Streptomyces coelicolor; venezuelae; morphological differentiation; WhiH; WhiA;

    Abstract : Streptomycetes are Gram-positive bacteria with a complex developmental life cycle. They form spores on specialized cells called aerial hyphae, and this sporulation involves alterations in growth, morphogenesis and cell cycle processes like cell division and chromosome segregation. READ MORE