Search for dissertations about: "cell factories"

Showing result 1 - 5 of 52 swedish dissertations containing the words cell factories.

2. 1. Droplet microfluidics for screening and sorting of microbial cell factories

   Author : Sara Björk; Helene Svahn Andersson; Håkan Jönsson; Christoph Merten; KTH; []
   Keywords : Droplet microfluidics; Cell factories; High-throughput screening; Cell culture; Bioteknologi; Biotechnology;

   Abstract : Cell factories are cells that have been engineered to produce a compound of interest, ranging from biopharmaceuticals to biofuels. With advances in metabolic engineering, the number of cell factory variants to evaluate has increased dramatically, necessitating screening methods with increased throughput. READ MORE

4. 2. Droplet microfluidics for single cell and nucleic acid analysis

   Author : Prem Kumar Periyannan Rajeswari; Helene Andersson Svahn; Håkan Jönsson; Martin Dufva; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Acoustophoresis; Biomarker detection; Cell behavior analysis; Cell factories; Droplet microfluidics; Droplet PCR; High throughput biology; Label-free enrichment; Single cell analysis; Bioteknologi; Biotechnology;

   Abstract : Droplet microfluidics is an emerging technology for analysis of single cells and biomolecules at high throughput. The controlled encapsulation of particles along with the surrounding microenvironment in discrete droplets, which acts as miniaturized reaction vessels, allows millions of particles to be screened in parallel. READ MORE

5. 3. Next generation tools for microbial cell factories

   Author : Alister James Cumming; Daniel Daley; Neil Dixon; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Microbial cell factories; Recombinant protein production; Bacteria; Plasmid; Genetic sensor; Inducible promoter; Genetic cassette; biokemi; Biochemistry;

   Abstract : The bacterium E. coli is a popular choice for expressing recombinant proteins. It is easy to culture and many molecular tools for are available for directing protein production. When producing recombinant proteins, high yields and good quality soluble products are desirable. READ MORE

6. 4. Towards membrane engineering as a tool in cell factory design: A case study on acetic acid tolerance in Saccharomyces cerevisiae

   Author : Lina Lindahl; Chalmers tekniska högskola; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; butanol; inhibitors; lipidomics; Key words: Lignocellulose; Zygosaccharomyces bailii; molecular dynamics simulations; membrane permeability; carbon-14 uptake; ethanol; robustness;

   Abstract : The sustainable production of fuels, chemicals, and materials using renewable resources is a necessity if we are to reduce our ecological footprint and the rate of climate change. Lignocellulosic biomass, the major constituent of plant cell walls, is a renewable raw material with great potential due to its high abundance. READ MORE

7. 5. Strength in Numbers – Droplet Microfluidics for Multicellular Ensemble Applications

   Author : Martin Trossbach; Aman Russom; Håkan Jönsson; Nicole Pamme; KTH; []
   Keywords : Droplet Microfluidics; High-Throughput Screening; Microtissues; Microreactors; Cell Factories; Cell Culture; Biomaterials; Spheroids; Biotechnology; Bioteknologi;

   Abstract : The work presented in this doctoral thesis explores multicellular, biological, and biotechnological applications for microfluidic droplets, making use of a number of unique features of these miniaturized, highly scalable reaction vessels.Droplet microfluidics specializes in pico- to nanoliter sized aqueous droplets in an immiscible oil phase, borrowing techniques from the field of microfluidics, namely fluid actuation, detection systems and electronic peripherals. READ MORE