Search for dissertations about: "cellular thermal shift assay"

Showing result 1 - 5 of 7 swedish dissertations containing the words cellular thermal shift assay.

  1. 1. Proximity Extension Assay : a universal tool for proteomics in precision medicine

    Author : Phathutshedzo M. Muthelo; Ulf Landegren; Jochen Schwenk; Uppsala universitet; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Proximity Extension Assay; Precision medicine; Assay development; Biology with specialization in Molecular Biology; Biologi med inriktning mot molekylärbiologi;

    Abstract : Precision medicine aims to utilise biomarkers to ensure the right treatment is given to the right patient at the right time. In pursuit of this goal, sensitive protein detection is essential to improve opportunities for discovery, verification, validation and development of valuable protein biomarkers. READ MORE

  2. 2. Molecular Approaches to Explore Drug-Target Interactions

    Author : Abdullah Al-Amin; Ulf Landegren; Christopher Schofield; Uppsala universitet; []
    Keywords : Drug discovery; target engagement; target engagement-mediated amplification; cellular thermal shift assay; proximity extension assay; in situ PLA; high-content imaging; Molekylär medicin; Molecular Medicine;

    Abstract : Improved means to assess the clinical potential of drug candidates can critically influence development of new therapeutic entities, a central aim in medical life science. Drug discovery and development relies on construction and selection of small organic compounds or biological agents that bind targets of interest. READ MORE

  3. 3. Determining Ligand- and Lipid- Interactions of SLC Transporters

    Author : Yurie Chatzikyriakidou; David Drew; Robert Fredriksson; Stockholms universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Solute carrier transporter; nucleotide-sugar transporter; SLC35; sodium proton exchanger; SLC9B2; thermal stability assay; membrane transport; Synaptic vesicle protein 2; Biochemistry; biokemi;

    Abstract : Solute carrier transporters (SLCs) mediate the inter- and intra- cellular trafficking of a plethora of substrates and are essential to cell homeostasis. Despite their importance to human physiology and their potential as therapeutic targets, many SLCs are considered orphans as the physiological substrate has not been experimentally determined. READ MORE

  4. 4. Targeting polo-like kinase 1 in pediatric leukemia

    Author : Oksana Goroshchuk; Karolinska Institutet; Karolinska Institutet; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP;

    Abstract : Acute lymphoblastic leukemia (ALL) is one of the most common cancers among children in the world, with almost 90% complete remission rate after the primary treatment. However, some patients are resistant to treatment and some relapse later. Moreover, existing treatments are toxic and induce life-long adverse effects. READ MORE

  5. 5. Mechanisms of cancer cell death by mutant p53-reactivating compound APR-246

    Author : Sophia Ceder; Karolinska Institutet; Karolinska Institutet; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP;

    Abstract : Tumor suppressor TP53 is the most frequently mutated gene in cancer. A majority of TP53 mutations result in a mutant p53 that disrupts its DNA binding capabilities but may also acquire novel gain-of-function activities that contribute to tumor growth. READ MORE