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Showing result 1 - 5 of 8 swedish dissertations matching the above criteria.

  2. 1. Functional studies and engineering of family 1 carbohydrate-binding modules

    Author : Janne Lehtiö; KTH; []
    Keywords : Cellulose-binding; carbohydrate-binding modules; phage display; bacterial surface display; combinatorial protein library; protein engineering; Trichoderma reesei; Staphylococcus carnosus;

    Abstract : The family 1 cellulose-binding modules (CBM1) form a groupof small, stable carbohydrate-binding proteins. These modulesare essential for fungal cellulosedegradation. This thesisdescribes both functional studies of the CBM1s as well asprotein engineering of the modules for several objectives. READ MORE

  4. 2. Strategies for cellulose fiber modification

    Author : Per Persson; KTH; []
    Keywords : Candida antarctica lipase B; cationic catalysis; cellulose-binding module; dendrimer; enzymatic polymerization; fiber modification; silica-cast replica; sol-gel mineralization; organocatalysis; xyloglucan endotransglycosylase;

    Abstract : This thesis describes strategies for and examples ofcellulose fiber modification.The ability of an engineered biocatalyst, acellulose-binding module fused to theCandida antarcticalipase B, to catalyze ring-openingpolymerization of e-caprolactone in close proximity tocellulose fiber surfaces was explored. READ MORE

  5. 3. Design of enzymatic systems for the modification of cellulose fiber surfaces

    Author : Malin Gustavsson; KTH; []
    Keywords : Candida antarctica lipase B; Pichia pastoris; cellulose-binding module; proteolysis; carbohydrate ester; xyloglucan endotransglycosylase; fiber modification;

    Abstract : This thesis describes the design of enzymatic systems forthe modification of cellulose fiber surfaces. A single-enzymeapproach employed a conjugate between theNeocallimastix patriciarumCel6A cellulose-bindingmodule and lipase B fromCandida antarctica(CBM-CALB) to enhance theaccessibility of the enzyme to the target hydroxyl groups. READ MORE

  6. 4. Expression and Mutagenesis studies of Candida antactica lipase B

    Author : Johanna C. Rotticci-Mulder; KTH; []
    Keywords : Protein expression; Candida antarctica lipase B; Pichia pastoris; site-directed mutagenesis; fermentation; selection;

    Abstract : Recombinant Candida antarctica lipase B was successfullyproduced in the methylotropic yeast Pichia pastoris. Thespecific activities of Candida antarctica lipase B produced inPichia pastoris and commercial Candida antarctica lipase B fromNovozymes were the same. In shake-flask cultivations theexpression levels were about 25 mg L-1. READ MORE

  7. 5. Structure and Function of Beta-Mannanases from Glycoside Hydrolase Clan A. A Study of Hemicellulases from Microbes and a Mollusc

    Author : Lars Anderson; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Proteiner; enzymologi; enzymology; Proteins; subsite organisation; transglycosylation; enzyme kinetics; beta-mannanase; hemicellulose;

    Abstract : Hemicellulose is the second most abundant group of renewable polysaccharides. Mannans can be found as the major hemicellulose in softwood and as storage polysaccharides in a range of plants. In this work, structural and functional characterisations of family 5 and 26 endo-1,4-b-mannanases (b-mannanases) have been performed. READ MORE