Search for dissertations about: "charged residues"

Showing result 1 - 5 of 87 swedish dissertations containing the words charged residues.

  2. 1. Conformational properties of transmembrane polypeptide segments in the ER membrane

    Author : IngMarie Nilsson; Karolinska Institutet; Karolinska Institutet; []
    Keywords : glycosylation; membrane protein; transmembrane helix; proline; charged residues;

    Abstract : We have developed a new experimental approach where the active site of oligosaccharyl transferase is used as a point of reference against which the position of a transmembrane segment in the membrane can be measured. This so-called glycosylation mapping technique allows any transmembrane segment to be positioned relative to a known reference transmembrane helix with known position in the membrane. READ MORE

  4. 2. Single-chain antibody construction and functional mapping of the monoclonal antibody TS1 : Its interaction with the antigen and the anti-idiotype

    Author : Patrik Holm; Birgitta Sundström; Per-Åke Nygren; Karlstads universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Chemistry; Kemi; Chemistry; Kemi; Biomedicin; Biomedical Laboratory Science;

    Abstract : The aims of this study are to synthesize and produce a single-chain antibody (scFv) of the anti-cytokeratin 8 monoclonal IgG antibody TS1 and to functionally map amino acid residues important for the interaction with its antigen and the anti-idiotypic antibody TS1. The TS1 antibody has been shown to be effective in binding cytokeratin 8 (CK8) expressed in tumors in vivo and is proposed to be useful in immunotargeting and/or immunotherapy. READ MORE

  5. 3. Exploring molecular interactions between polypeptide conjugates and protein targets : Manipulating affinity by chemical modifications

    Author : Aleksandra Balliu; Lars Baltzer; Oliver Seitz; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; polypeptide conjugates; protein targets; molecular recognition; affinity enhancement; conjugation;

    Abstract : In this thesis molecular interactions between polypeptide conjugates and protein targets were investigated. Polypeptides were derivatized with small organic molecules, peptides and oligonucleotides. New strategies were developed with the aim to increase affinities for proteins of biological interest. READ MORE

  6. 4. Anisotropic Protein Interactions in Salt Solutions and at Interfaces: Coarse Grained Modeling

    Author : Anil Kurut Sabanoglu; Beräkningskemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Anisotropic interactions; protein electrostatics; phase association; surface adsorption; coarse grained models; Monte Carlo simulations;

    Abstract : Anisotropic protein interactions have a strong orientation dependence resulting from an uneven distribution of charged and hydrophobic residues on the protein surface. They play an important role in protein behaviors such as protein association, surface adsorption and phase separation. READ MORE

  7. 5. Transmembrane helices : tails, turns and snorkels

    Author : Magnus Monné; Donald Engelman; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; biokemi; Biochemistry;

    Abstract : The assembly and structure of membrane proteins are intimately related. Targeting and insertion of membrane proteins into the endoplasmatic reticulum (ER) membrane involve many components that facilitate the folding of the native structure. READ MORE