Search for dissertations about: "circular dichroism"

Showing result 11 - 15 of 115 swedish dissertations containing the words circular dichroism.

  2. 11. Protein production and purification in structural genomics

    Author : Martin Hammarström; Per-Åke Nygren; Torleif Härd; Helena Berglund; Herman van Tilbeurgh; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Recombinational cloning; Escherichia coli; gene expression; fusion proteins; solubility; circular dichroism; nuclear magnetic resonance; Structural biochemistry; Strukturbiokemi;

    Abstract : The number of gene products available for structural and functional study is increasing at an unprecedented rate as a result of the successful whole genome sequencing projects. Systematic structure determination of proteins on a genomic scale, called structural genomics, can significantly contribute to the field of protein science and to functional annotation of newly identified genes. READ MORE

  4. 12. A Chloroplast Localized Small Heat Shock Protein, Hsp21. Importance of Hsp21 in stress protection of transgenic Arabidopsis thaliana, recombinant expression and characterization of oxidation-dependent conformational changes

    Author : Ulrika Härndahl; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; a-crystallin; sHsp; chaperone; amphipathic a-helix; oligomer; redox-regulated; methionine sulfoxidation; oxidative stress; regulation; protein structure; circular dichroism; mass spectrometry; fluorescence spectroscopy; Biochemistry; Metabolism; Biokemi;

    Abstract : A specific group of heat shock proteins, the so called small heat shock proteins (sHsps), exists in all organisms but are especially abundant in plants. The sHsps prevent aggregation of other proteins (substrate proteins) during transient heat and oxidative stress. READ MORE

  5. 13. Self-assembly of amyloid-β peptides in the presence of metal ions and interacting molecules – a detour of amyloid building blocks

    Author : Cecilia Mörman; Astrid Gräslund; Daniel Otzen; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; biophysics; Alzheimer’s disease; protein aggregation; amyloid formation; amyloid-β peptide; aggregation kinetics; interactions; metal ions; designed peptide constructs; Tau protein; NMR; circular dichroism; fluorescence spectroscopy; Biophysics; biofysik;

    Abstract : Misfolding of proteins into amyloid structures is implicated as a pathological feature in several neurodegenerative diseases and the molecular causes are still unclear. One typical characteristic of Alzheimer’s disease is self-assembly and accumulation of soluble amyloid-β (Aβ) peptides into insoluble fibrils and plaques. READ MORE

  6. 14. Coarse-grained and atomistic modelling of phosphorylated intrinsically disordered proteins

    Author : Ellen Rieloff; Beräkningskemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; intrinsically disordered proteins; phosphorylation; simulations; Monte Carlo; molecular dynamics; coarse-graining; atomistic; statherin; small-angle X-ray scattering; circular dichroism;

    Abstract : Intrinsically disordered proteins (IDPs) are involved in many biological processes such as signalling, regulation and recognition. One of the main questions regarding IDPs is how sequence, structure and function are related. READ MORE

  7. 15. Nucleic Acids as Drug Targets -Interactions of Platinum Complexes, Polyamines, and siRNA with DNA and RNA Models

    Author : Margareta Hägerlöf; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Kinetics; Kemi; Chemistry; AU-rich region; siRNA; Circular Dichroism; Polyamine; Modified Nucleoside; HPLC; Thermodynamic Stability; cisplatin; Hairpin; DNA and RNA conformation; RNA; DNA; Anticancer Drug; Platinum Complexes;

    Abstract : Drugs targeting nucleic acids were studied in DNA and RNA model systems. The effects were investigated with respect to structural and thermodynamic influence, kinetics, and biological function. The drugs employed in this thesis were anticancer active platinum(II) complexes, polyamine derivatives, and siRNA. READ MORE