Search for dissertations about: "clone"

Showing result 1 - 5 of 201 swedish dissertations containing the word clone.

2. 1. Microwell devices for single-cell analyses

   Author : Sara Lindström; Helene Andersson-Svahn; Mehmet Toner; KTH; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; microwell; miniaturization; microfluidics; cell culture; single-cell; clone; imaging; stem cell; cancer; low volume; high-throughput; Molecular biology; Molekylärbiologi;

   Abstract : Powerful tools for detailed cellular studies are emerging, increasing the knowledge ofthe ultimate target of all drugs: the living cell. Today, cells are commonly analyzed inensembles, i.e. thousands of cells per sample, yielding results on the average responseof the cells. READ MORE

4. 2. Miniaturised Microwell-based Cell Assays

   Author : Emilie Weibull; Helene Andersson Svahn; Albert van den Berg; KTH; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; microwell; miniaturisation; high-resolution imaging; high-throughput; cell culture; single-cell; clone; heterogeneity; antibiotic susceptibility testing; concentration gradient; interfacing; microchannels; cell retrieval;

   Abstract : Cell heterogeneity in genetically identical cell populations is becoming a well-known and important phenomenon in cell biology. Current methods commonly utilise population-based analysis founded on averaged result. Hence there is a need for high-throughput cell assays on the single-cell level. READ MORE

5. 3. Recombinant Enzymes in Pyrosequencing Technology

   Author : Nader Nourizad; KTH; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; apyrase; pyrosequencing; pichia pastoris; protein expression; fusion protein; luciferase; DNA template; clone checking; DNA polymerase;

   Abstract : Pyrosequencing is a DNA sequencing method based on thedetection of released pyrophosphate (PPi) during DNA synthesis.In a cascade of enzymatic reactions, visible light isgenerated, which is proportional to the number of nucleotidesincorporated into the DNA template. When dNTP(s) areincorporated into the DNA template, inorganic PPi is released. READ MORE

6. 4. Genetic variation, clonal diversity and breeding system in sedges (Carex)

   Author : Olle Jonsson; Biologiska institutionen; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Systematic botany; Carex ericetorum; Carex caryophyllea; Carex bigelowii; Carex arenaria; phenology; niche; genet identification; clone size; isozyme; gene flow; introgression; allozyme; gene diversity; population genetics; taxonomy; morphology; phytogeography; chemotaxonomy. Physiology of nonvascular plants; Systematisk botanik; taxonomi; morfologi; kemotaxonomi. Växters fysiologi inte kärlväxter .;

   Abstract : In this thesis I investigate genetic variation in clonal plants within the wind-pollinated plant family Cyperaceae and, in particular, in species of Carex. Clonal diversity and the spatial distribution of clones (genets) within populations was investigated in Carex bigelowii (in Iceland and Scandinavia) and C. READ MORE

7. 5. Genetics and ecology of natural populations

   Author : Elisabeth Lundqvist; Ulf Lagercrantz; Umeå universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Angelica archangelica; Bistorta vivipara; Viscaria alpina; Eiseniella tetraedra; ecotypes; hydrochory; genetic differentiation; clone diversity; genetic patterns; riparian;

   Abstract : I have studied the genetic variation of single species using morphological variation and enzyme electrophoresis. I have striven to understand the interaction between the breeding structure and the ecology of the species in relation to the community, in which it lives. The work was done in the county of Västerbotten, northern Sweden. READ MORE