Search for dissertations about: "clustered DNA damage"

Showing result 1 - 5 of 8 swedish dissertations containing the words clustered DNA damage.

  2. 1. Radiation response in human cells : DNA damage formation, repair and signaling

    Author : Ann-Sofie Gustafsson; Bo Stenerlöw; Andrzej Wojcik; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; DNA damage; DNA repair; DSB; NHEJ; DNA-PK; ionizing radiation; heat-labile sites; Biomedical Radiation Science; Biomedicinsk strålningsvetenskap;

    Abstract : Ionizing radiation induces a range of different DNA lesions. In terms of mutation frequency and mammalian cell survival, the most critical of these lesions is the DNA double-strand break (DSB). DSB left unrepaired or mis-repaired may result in chromosomal aberrations that can lead to permanent genetic changes or cell death. READ MORE

  4. 2. Induction and repair of clustered DNA damage sites after exposure to ionizing radiation

    Author : Andris Abramenkovs; Bo Stenerlöw; Peter O'Neill; Uppsala universitet; []
    Keywords : NHEJ; DSB repair; clustered DNA damage; DNA repair; DNA-PKcs; HSCS; Ra-223; ionizing radiation; Medicinsk vetenskap; Medical Science;

    Abstract : The mechanisms that maintain genomic stability safeguard cells from constant DNA damage produced by endogenous and external stressors. Therefore, this thesis aimed to specifically address questions regarding the requirement and involvement of DNA repair proteins in the repair of various types of radiation-induced DNA damage. READ MORE

  5. 3. Role of Non-Homologous End-Joining in Repair of Radiation-Induced DNA Double-Strand Breaks

    Author : Karin Karlsson; Bo Stenerlöw; Jörgen Carlsson; Thomas Helleday; Uppsala universitet; []
    Keywords : Molecular biology; DNA damage; DNA repair; DSB; NHEJ; DNA-PK; ionising radiation; high-LET; heat-labile sites; PFGE; Molekylärbiologi;

    Abstract : Efficient and correct repair of DNA damage, especially DNA double-strand breaks (DSBs), is vital for the survival of individual cells and organisms. Defects in the DNA repair may lead to cell death or genomic instability and development of cancer. READ MORE

  6. 4. Formation and repair of complex DNA damage induced by ionizing radiation

    Author : Karin Magnander; Göteborgs universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ionizing radiation; clustered damage; chromatin structure; DSB;

    Abstract : DNA is the critical target when cells are exposed to ionizing radiation, a potent stressor with capacity to produce complex DNA damages, thereby increasing the risk of cancer. DNA and associated histones form chromatin, which is an effective protection against ionizing radiation. READ MORE

  7. 5. Improving integration efficiency and precision of CRISPR/Cas9-mediated genome editing

    Author : Sandra Wimberger; Göteborgs universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Genome editing; CRISPR-Cas9; DNA repair; genomic integration; off-targets;

    Abstract : The clustered regularly interspaced short palindromic repeats (CRISPR) – CRISPR-associated protein 9 (Cas9) system has revolutionized the field of genome engineering, providing a cost-effective and fast tool for targeted gene modifications. Endogenous repair pathways, including error-prone non-homologous end joining and alternative end joining, or precise homology-directed repair (HDR), mend Cas9-induced DNA double-strand breaks. READ MORE