Search for dissertations about: "combinatorial library"

Showing result 16 - 20 of 54 swedish dissertations containing the words combinatorial library.

  2. 16. Functional studies and engineering of family 1 carbohydrate-binding modules

    Author : Janne Lehtiö; KTH; []
    Keywords : Cellulose-binding; carbohydrate-binding modules; phage display; bacterial surface display; combinatorial protein library; protein engineering; Trichoderma reesei; Staphylococcus carnosus;

    Abstract : The family 1 cellulose-binding modules (CBM1) form a groupof small, stable carbohydrate-binding proteins. These modulesare essential for fungal cellulosedegradation. This thesisdescribes both functional studies of the CBM1s as well asprotein engineering of the modules for several objectives. READ MORE

  4. 17. Dynamic Systems for Screening, Control and Identification of Protein-Ligand Interactions

    Author : Rikard Larsson; Olof Ramström; Michel R. Gagné; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Dynamic combinatorial chemistry; Dynamic kinetic combinatorial resolution; Catalytic screening; Transthiolesterification; Thiol disulfide interchange; Nitroaldol reaction; Lectins; Hydrolases; Dynamic self-inhibition; Organic chemistry; Organisk kemi;

    Abstract : Dynamic systems for screening, control and identification of different protein-ligand interactions are presented. Dynamic chemistry is used to produce new compounds/constituents in situ that can interact with a target molecule. Several entities can be introduced at the same time and interact with one another. READ MORE

  5. 18. Development of molecular recognition by rational and combinatorial engineering

    Author : Andreas Jonsson; Stefan Ståhl; Mats Persson; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Affibody molecule; albumin binding domain; protein engineering; phage display; amyloid beta peptide; TNF; HSA; Molecular biology; Molekylärbiologi;

    Abstract : Combinatorial protein engineering, taking advantage of large libraries of protein variants and powerful selection technology, is a useful strategy for developing affinity proteins for applications in biotechnology and medicine. In this thesis, two small affinity proteins have been subjected to combinatorial protein engineering to improve or redirect the binding. READ MORE

  6. 19. Engineering of small IgG binding domains for antibody labelling and purification

    Author : Sara Kanje; Sophia Hober; Cecília Roque; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Antibody; labelling; purification; Protein G; Protein A; protein engineering; protein design; combinatorial selection; Bioteknologi; Biotechnology;

    Abstract : In protein engineering, rational design and selection from combinatorial libraries are methods used to develop proteins with new or improved features. A very important protein for the biological sciences is the antibody that is used as a detecting agent in numerous laboratory assays. READ MORE

  7. 20. Combinatorial protein engineering applied to enzyme catalysis and molecular recognition

    Author : Malin Eklund; KTH; []
    Keywords : phage display; selection; enzyme; affinity domains; crystal structure; affibody; lipase; protein engineering; protein A; assembly;

    Abstract : The recent development of methods for constructing andhandling large collections (libraries) of proteins, from whichvariants with desired traits can be isolated, hasrevolutionized the field of protein engineering. Key elementsof such methods are the various ways in which the genotypes(the genes) and the phenotypes (the encoded proteins) arephysically linked during the process. READ MORE