Search for dissertations about: "combinatorial protein engineering"

Showing result 21 - 25 of 44 swedish dissertations containing the words combinatorial protein engineering.

  2. 21. Affibody molecules for proteomic and therapeutic applications

    Author : Caroline Grönwall; Stefan Ståhl; Lena Jendeberg; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Affibody; protein engineering; phage display; amyloid beta peptide; transferrin; CD25; IL-2 receptor; proteomics; Bioengineering; Bioteknik;

    Abstract : This thesis describes generation and characterization of Affibody molecules with future applications in proteomics research, protein structure determinations, therapeutic treatment of disease and medical imaging for in vivo diagnostics. Affibody molecules are engineered affinity proteins developed by combinatorial protein engineering from the 58-residue protein A-derived Z domain scaffold. READ MORE

  4. 22. Affibody molecules targeting the epidermal growth factor receptor for tumor imaging applications

    Author : Mikaela Friedman; Stefan Ståhl; Fredrik Nilsson; James S. Huston; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Affibody; affinity maturation; phage display selection; EGFR; molecular imaging; protein engineering; tumor targeting; Bioengineering; Bioteknik;

    Abstract : Tumor targeting and molecular imaging of protein markers specific for or overexpressed in tumors can add useful information in deciding upon treatment and assessing the response to treatment for a cancer patient. The epidermal growth factor receptor (EGFR) is one such tumor-associated receptor, which expression is abnormal or upregulated in various cancers and associated with a poor patient prognosis. READ MORE

  5. 23. Engineering alternative scaffold proteins for conditional targeting

    Author : Malin Jönsson; Sophia Hober; Mark Howarth; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Protein Engineering; Conditional targeting; Calcium; Biotechnology; Bioteknologi;

    Abstract : Engineering naturally occurring proteins enables us to customize affinity domains according to our specific needs, tailoring them to become an important tool in a wide array of applications limited merely by our creativity. One of nature’s ways to regulate protein activity is by creating a functional change through alteration of a protein’s tertiary structure upon interaction with metal ions. READ MORE

  6. 24. Chemical Synthesis of Affibody Molecules for Protein Detection and Molecular Imaging

    Author : Torun Ekblad; Amelie Eriksson Karlström; Per-Åke Nygren; Harald Kolmar; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Affibody; peptide synthesis; protein detection; molecular imaging; Bioengineering; Bioteknik;

    Abstract : Proteins are essential components in most processes in living organisms. The detection and quantification of specific proteins can be used e.g. as measures of certain physiological conditions, and are therefore of great importance. READ MORE

  7. 25. Staphylococcal surface display in directed evolution

    Author : Nina Kronqvist; Stefan Ståhl; Mats A. A. Persson; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; affibody; combinatorial library; directed evoluation; Gram-positive bacteria. protein engineering; Bioengineering; Bioteknik;

    Abstract : Engineered affinity proteins have together with naturally derived antibodies becomeindispensable tools in many areas of life-science and with the increasing number ofapplications, the need for high-throughput methods for generation of such different affinityproteins is evident. Today, combinatorial protein engineering is the most successful strategy toisolate novel non-immunoglobulin affinity proteins. READ MORE