Search for dissertations about: "comet assay"
Showing result 11 - 15 of 27 swedish dissertations containing the words comet assay.
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11. Effects of Antioxidants and Pro-oxidants on Oxidative Stress and DNA Damage using the Comet Assay : Studies on Blood Cells from Type 2 Diabetes Subjects and Mouse Lymphoma Cells
Abstract : Diet and oral supplements comprise two distinct sources of antioxidants known to prevent oxidative stress. Beneficial effects from antioxidants have been seen for patients at risk for type 2 diabetes.The aim of this thesis was to evaluate the positive effects of antioxidants against oxidative stress and DNA damage in type 2 diabetes subjects. READ MORE
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12. International validation of the comet assay and a human intervention study
Abstract : The comet assay is an established, sensitive method extensively used in biomonitoring studies. The methods’ advantages include; a) only a small cell sample is required, b) possibility to measure damage in practically any cell type, c) ability to measure heterogeneity in response within a cell population, d) relatively fast and economical procedure, and e) various applications of the method, which allow measurement of a range of different DNA lesions as well as DNA repair. READ MORE
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13. Measurement of primary DNA damage in individual cells from humans and mice using the alkaline single cell gel electrophoresis assay
Abstract : Primary DNA damage is considered to be an important step in the development of tumours and itmay also serve as a biomarker of genotoxic stress. Measurement of DNA strand breaks in peripherallymphocytes can yield useful information about the suitability of these cells for biomonitoring purposesand subsequently risk assessment. READ MORE
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14. DNA repair and genome organisation in mammalian cells : studies involving single-cell electrophoresis
Abstract : The comet assay (single-cell electrophoresis) is a comparably new method for measurement of DNA strand breakage, primarily in eukaryotic cells. Its main advantage is that it works on the single-cell level, making it possible to detect heterogeneity in DNA damage induction and repair. READ MORE
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15. Induction of apoptosis in relation to chromatin structure and inhibition of replication by DNA damage from ionizing radiation
Abstract : The theme of this thesis has been chromatin organization ranging from methodological studies to involvement in apoptotic response. The aims have been: (i) to compare the information obtained by the AVTD method and comet assay concerning DNA-loop organization, (ii) to test the hypothesis that DNA-loop relaxation could be the triggering signal for induction of apoptosis in G0-lymphocytes, and (iii) to study the dose response for inhibition of the replication fork movement and pathways for the DNA repair process at the replication fork. READ MORE