Search for dissertations about: "complementary DNA"

Showing result 1 - 5 of 82 swedish dissertations containing the words complementary DNA.

2. 1. DNA-Controlled Lipid-Membrane Fusion

   Author : Lisa Simonsson; Chalmers tekniska högskola; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; exocytosis; vesicles; DNA; FRET; fluorescence resonance energy transfer; model system; total internal reflection fluorescence microscopy; TIRF microscopy; self-assembly; site-specificity; diffusion; biomembranes; lipid bilayer; membrane fusion;

   Abstract : Membrane fusion is essential for nerve-cell communication, for protein transport between cell organelles and the cell-membrane and for enabling the merger between virus and host membranes during virus infection. We have demonstrated that short DNA oligonucleotides, membrane-attached via CH in an orientation that mimics the overall zipperlike architecture of fusion-inducing proteins, induce fusion of both suspended vesicles and vesicles site-specifically tethered to SLBs. READ MORE

4. 2. Structural Adaptation of DNA to Small Ligands and Recombination Proteins

   Author : Hye-Kyung Kim; Chalmers tekniska högskola; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; triplex DNA; groove binders; intercalators; SANS; DNA; optical spectroscopy; recombination proteins;

   Abstract : In order to contribute to the understanding of mechanisms of recognition in nucleic acid systems, the interaction of DNA with a variety of ligands, representing different cases of adaptation of the double-helix structure, was studied using flow linear dichroism (LD), circular dichroism (CD), fluorescence and absorption spectroscopic techniques. A chiral hydrophobic metal complex, [Ru(dpphen)3]2+ was examined, since this had been reported to be the most efficient molecule to discriminate DNA handedness. READ MORE

5. 3. DNA Analysis of PCR-Inhibitory Forensic Samples

   Author : Johannes Hedman; Teknisk mikrobiologi; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; amylase; statistical modelling; principal components analysis; PCR inhibitors; PCR inhibition; forensic DNA analysis; DNA polymerase blend; DNA polymerase; crime scene samples;

   Abstract : DNA evidence, linking perpetrators to crime scenes, is central to many legal proceedings. However, crime scene samples often contain extraneous substances that may interfere with the PCR-based forensic analysis, resulting in partial or negative DNA profiles. Extensive DNA purification may remove inhibitors, but involves the risk of DNA loss. READ MORE

6. 4. Sensitive Identification Tools in Forensic DNA Analysis

   Author : Hanna Edlund; Marie Allen; Niels Morling; Uppsala universitet; []
   Keywords : forensic genetics; STRs; Y-chromosome; Pyrosequencing; mitochondrial DNA; skeletal remains; DNA extraction; MEDICINE; MEDICIN; Medicinsk genetik; Medical Genetics;

   Abstract : DNA as forensic evidence is valuable in criminal investigations. Implementation of new, sensitive and fast technologies is an important part of forensic genetic research. This thesis aims to evaluate new sensitive methods to apply in forensic DNA analysis including analysis of old skeletal remains. READ MORE

7. 5. Unravelling Mechanistic Insights of Eukaryotic Transcriptional Control

   Author : Johanna Hörberg; Göteborgs universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Transcriptional control; Gene expression regulation; DNA supercoiling; DNA methylation; DNA-protein recognition; Basic-Leucine-Zipper transcription factors; Basic-Helix-Loop-Helix transcription factors; Sequence specific DNA plasticity; Transcription factor cooperativity; Cancer; Molecular modelling; Molecular dynamics;

   Abstract : DNA transcription involves the association of multiple proteins with DNA to convert the genetic information into messenger-RNA transcripts, which later will be translated into proteins. Con-trolled regulation of transcription is imperative for preserving the healthy and homeostatic state of cells. READ MORE