Search for dissertations about: "coupled folding"

Showing result 1 - 5 of 22 swedish dissertations containing the words coupled folding.

2. 1. Protein Folding Studied by Site Directed Mutagenesis

   Author : Tomas Ternström; Biokemi och Strukturbiologi; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Metabolism; Biokemi; metabolism; Biochemistry; Transition state; Activation Barrier; Two-State; Protein Folding; Protein Engineering;

   Abstract : The N-terminal RNA binding domain of U1A has been shown to fold in a two-state process without accumulating any intermediates. U1A has a symmetrically curved chevron plot which seems to result mainly from changes of the transition state. READ MORE

4. 2. The Folding Energy Landscape of MerP

   Author : Ann-Christin Brorsson; Bengt-Harald Jonsson; Daniel Otzen; Umeå universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; protein folding and stability; hydrogen exchange; intermediate; partial unfolding; Biokemi; Biochemistry; Biokemi; Biochemistry; biokemi;

   Abstract : This thesis is based on studies, described in four papers, in which the folding energy landscape of MerP was investigated by various techniques. MerP is a water-soluble 72 amino acid protein with a secondary structure consisting of four anti-parallel β-strands and two α-helices on one side of the sheet in the order β1α1β2β3α2β4. READ MORE

5. 3. Structure determination and thermodynamic stabilization of an engineered protein-protein complex

   Author : Elisabet Wahlberg; Per-Åke Nygren; Bengt-Harald Jonsson; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; affibody; protein structure; coupled folding; NMR spectroscopy; protein stability; protein-protein interactions; binding thermodynamics; isothermal titration calorimetry; protein engineering; Structural biochemistry; Strukturbiokemi;

   Abstract : The interaction between two 6 kDa proteins has been investigated. The studied complex of micromolar affinity (Kd) consists of the Z domain derived from staphylococcal protein A and the related protein ZSPA-1, belonging to a group of binding proteins denoted affibody molecules generated via combinatorial engineering of the Z domain. READ MORE

6. 4. Structural Transitions in Helical Peptides : The Influence of Water – Implications for Molecular Recognition and Protein Folding

   Author : Martin Lignell; Hans-Christian Becker; Kaare Teilum; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; protein dynamics; protein folding; molten globule; time-resolved fluorescence spectroscopy; CD spectroscopy; molecular recognition; structure-function paradigm; Chemical physics; Kemisk fysik; Physical Chemistry; Fysikalisk kemi;

   Abstract : Fluctuations in protein structure are vital to function. This contrasts the dominating structure-function paradigm, which connects the well-defined three-dimensional protein structure to its function. However, catalysis is observed in disordered enzymes, which lack a defined structure. READ MORE

7. 5. Protein folding studies of human superoxide dismutase and ALS associated mutants

   Author : Mikael Lindberg; Mikael Oliveberg; Jane Clarke; Umeå universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; superoxide dismutase; ALS; protein folding; equilibrium titration; protein stability; apo protein; disulphide bridge; chevron plot; Biokemi; Biochemistry; Biokemi; Biochemistry; biokemi;

   Abstract : Proteins are among the most abundant biological macromolecules. The cellular machinery is coupled to exact structural shape and properties of the more than 100 000 different proteins. Still, proteins can sometimes completely change their character and as a result trigger neuro degenerative disease. READ MORE