Search for dissertations about: "crıspr"

Showing result 1 - 5 of 99 swedish dissertations containing the word crıspr.

  2. 1. The type I-E CRISPR-Cas system : Biology and applications of an adaptive immune system in bacteria

    Author : Lina Amlinger; Magnus Lundgren; Peter Fineran; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; CRISPR; CRISPR-Cas; virus defense; bacteria; bacteriophage; adaptation; spacer integration; interference; gene silencing; fluorescent reporter; Escherichia coli; Mikrobiologi; Microbiology;

    Abstract : CRISPR-Cas systems are adaptive immune systems in bacteria and archaea, consisting of a clustered regularly interspaced short palindromic repeats (CRISPR) array and CRISPR associated (Cas) proteins. In this work, the type I-E CRISPR-Cas system of Escherichia coli was studied.CRISPR-Cas immunity is divided into three stages. READ MORE

  4. 2. Advancing CRISPR technologies to engineer yeast metabolism

    Author : Raphael Ferreira; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; oleochemicals; metabolic engineering; Saccharomyces cerevisiae; CRISPR;

    Abstract : The advent of genetic engineering tools has initiated an era of manipulating microorganisms for the production of valuable compounds for our society. Precise engineering of these microbes commonly requires introducing genetic modifications such as gene deletion, overexpression, and accurate regulation in order to enhance the production of the compound of interest. READ MORE

  5. 3. CRISPR based technologies for high-throughput metabolic engineering of yeast

    Author : Christos Skrekas; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; cloning; Saccharomyces cerevisiae; enrichment; metabolic engineering; selection; library; screening; CRISPR; directed evolution;

    Abstract : The yeast Saccharomyces cerevisiae is a commonly used microorganism for metabolic engineering applications since it has a very well-studied metabolism and it can be easily genetically modified. This led to its use as a cell factory for the production of a wide variety of industrially relevant chemicals such as fuels, cosmetics and food additives. READ MORE

  6. 4. Modeling Human Hematopoiesis Using the CRISPR/Cas9 System

    Author : Alexandra Bäckström; Avdelningen för molekylärmedicin och genterapi; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Hematopoiesis; Hematopoietic stem and progenitor cells; HSC regulation; CRISPR Cas9; Gene editing;

    Abstract : Hematopoietic stem cells (HSCs) have the ability to self-renew and to give rise to all blood cells of the different lineages, and are thereby responsible for the replenishment of blood cells throughout life. These cells are tightly regulated by extrinsic and intrinsic regulators, such as signals from the bone marrow microenvironment, complex transcription factor networks and epigenetic regulators. READ MORE

  7. 5. CRISPR Screens Identify Candidate Therapeutic Targets in Leukemia

    Author : Maria Rodriguez Zabala; Avdelningen för klinisk genetik; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Acute myeloid leukemia; CRISPR screen; leukemia stem cell; cell surface receptor; metabolism; immunotherapy; CXCR4; GLUT1; TFR1; H2-K1;

    Abstract : Acute myeloid leukemia (AML) is a complex hematological malignancy marked by proliferation of immature myeloid cells with a dismal 5-year survival. A major challenge is the persistence of leukemia stem cells (LSCs) after standard treatment, leading to relapse. READ MORE