Search for dissertations about: "cryo-transmission electron microscopy"

Showing result 1 - 5 of 12 swedish dissertations containing the words cryo-transmission electron microscopy.

2. 1. Groove-binding Unsymmetrical Cyanine Dyes for DNA Applications

   Author : Maja Eriksson; Chalmers tekniska högskola; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; polarized spectroscopy; bacteriophage T5; cryo-transmission electron microscopy; dynamic light scattering; DNA; intercalation; cyanine dyes; groove-binding; fluorescence spectroscopy; gel electrophoresis;

   Abstract : This work focuses on interactions between DNA and a group of newly developed unsymmetrical cyanine dyes. The aim of the work was to discover fluorescent dyes that can be used in biophysical studies of natural DNA where minimum perturbation of the DNA-helix is essential, where a slow dissociation rate of the dyes is needed and where the ionic strength is an important parameter. READ MORE

4. 2. Nanostructure and biomolecule interactions : Characterizing the complex

   Author : Stefan Gunnarsson; NanoLund: Centre for Nanoscience; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Nanostructures; Proteins; Lipids; Protein corona; Aggregation; Sedimentation; Diffusion; Electron microscopy; Enzyme activity; Nanowires; Nanoparticles;

   Abstract : This thesis presents the results of studies done on various biomolecules and their interactions with nanomaterials. The biomolecule sources are everything from purified, single proteins to the complicated mixture of blood serum and cell culture media. READ MORE

5. 3. Adsorption of proteins on zeolite FAU

   Author : Daniel Klint; Centrum för analys och syntes; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; matrix preparation; cryo-electron microscopy; protein purification; zeolites; Inorganic chemistry; Oorganisk kemi;

   Abstract : A highly dealuminated form of zeolite FAU, ultra-stable zeolite Y (USY), was used as an adsorbent matrix in the purification of proteins. Undesired proteins were removed from a crude preparation by adsorption on USY, increasing the purity of an unadsorbed protein (horse radish peroxidase), or the protein of interest was adsorbed on the zeolite and subsequently eluted through changes in the solution conditions (hen egg lysozyme, mouse-immunoglobulin G). READ MORE

6. 4. Association Phenomena in Aqueous Solutions of Surfactants and Hydrophobically Modified Polymers

   Author : Susanne Nilsson; Fysikalisk kemi; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; self-diffusion; rheology; cellulose ethers; associating polymers; polymer-surfactant interactions; polymer network; Fysikalisk kemi; Physical chemistry;

   Abstract : Interactions between ionic surfactants (sodium dodecyl sulfate and dodecyltrimethylammonium chloride) and nonionic polymers ((hydroxyethyl)cellulose, ethyl(hydroxyethyl)cellulose and their hydrophobically modified analogous) have been studied by means of rheology, pulsed field gradient nuclear magnetic resonance (PFG NMR), time-resolved fluorescence quenching (TRFQ), dynamic light scattering (DLS) and cryo-transmission electron microscopy (cryo-TEM). In all experiments surfactants have been added in various amounts to polymer solutions at fixed concentrations. READ MORE

7. 5. Structural Changes in Nonionic Surfactant Systems

   Author : Sanja Bulut; Fysikalisk kemi; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Vesicles; Nonionic Surfactant; Wormlike Micelles; Lamellar Phase; NMR; MRI; SLS; DLS; cryo-fracture-TEM; centrifugation; cryo-TEM;

   Abstract : In this thesis we have studied structural changes of a nonionic surfactant self-assembly, as governed by changes in temperature and concentration. The techniques used in experimental investigations were dynamic and static light scattering, nuclear magnetic resonance and cryo-transmission electron microscopy (using a novel specimen preparation protocol). READ MORE