Search for dissertations about: "cyanine dyes"

Showing result 1 - 5 of 13 swedish dissertations containing the words cyanine dyes.

  2. 1. Groove-binding Unsymmetrical Cyanine Dyes for DNA Applications

    Author : Maja Eriksson; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; polarized spectroscopy; bacteriophage T5; cryo-transmission electron microscopy; dynamic light scattering; DNA; intercalation; cyanine dyes; groove-binding; fluorescence spectroscopy; gel electrophoresis;

    Abstract : This work focuses on interactions between DNA and a group of newly developed unsymmetrical cyanine dyes. The aim of the work was to discover fluorescent dyes that can be used in biophysical studies of natural DNA where minimum perturbation of the DNA-helix is essential, where a slow dissociation rate of the dyes is needed and where the ionic strength is an important parameter. READ MORE

  4. 2. Detection of Biomolecules: Syntheses and Studies of Minor Groove Binding Unsymmetrical Cyanine Dyes and Chromogenic Enzyme Substrates

    Author : Jonas Karlsson; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; palladium-catalysed cross-couplings; optical spectroscopy; minor groove binding; DNA; cyanine dye; enzyme; intercalation; fluorescence; chromogenic;

    Abstract : Visualisation or detection of biomolecules is usually achieved by the use of fluorescent or highly coloured chromophores. The work presented in this thesis deals with the development of new chromogenic substrates for the detection of enzyme activity, and new DNA-binding fluorescent dyes for staining of DNA. READ MORE

  5. 3. Minor groove binding unsymmetrical cyanine dyes : binding and dissociation studies in free solution and in electrophoresis gels

    Author : Maja Eriksson; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; electrophoresis; cyanide dyes; dissociation kinetics; minor groove binding; DNA; stopped-flow; unwinding; mobility shift assays;

    Abstract : .... READ MORE

  6. 4. DNA-Photosensitization by New Drugs for Photoinactivation of Viruses and Phototherapy of Tumors

    Author : Claire Kanony; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; 7; hydroxyl radicals; singlet oxygen; 8-dihydro-8-oxo-deoxyguanosine; pyropheophorbides; electron transfer; DNA damage; hydrogen abstraction; cyanine dyes;

    Abstract : Cyanines and pyropheophorbides are two classes of photoactivable dyes which are particularly interesting for photosterilization of blood used in transfusion and for treatment of localised cancers by photodynamic therapy (PDT). After a bibliographic review about the principal mechanisms of DNA photosensitization, studies about the photosensitizing properties of these two families of compounds are presented. READ MORE

  7. 5. Chromophores for visualisation of nucleic acid recognition processes

    Author : Marcus Wilhelmsson; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; DNA base analogue; chromophore aggregation; DNA-PNA-duplex stability; genetic screening; cyanine dyes; flourescence quantum yield; PNA; FRET;

    Abstract : .... READ MORE