Search for dissertations about: "dUTPase"

Found 5 swedish dissertations containing the word dUTPase.

  2. 1. Nucleotide Binding to Trimeric dUTPase: Studies on Recombinant Enzymes using Kinetic and Spectroscopic Methods

    Author : Johan Nord; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; C-terminus; dUTPase; EIAV; Escherichia coli; flexibility; Homo sapiens; kinetics; NMR; nucleotide metabolism; rate constants of lig-and binding; production of recombinant enzyme; dUTP; specificity; Biochemistry; Metabolism; Biokemi;

    Abstract : Recombinant dUTPase from the bacterium Escherichia coli, the retrovirus equine infectious anemia virus (EIAV) and Homo sapiens have been made available for physico-chemical characterisation. The protocols developed have also proven to be suitable for production of enzymes modified by isotope labelling or other methods. READ MORE

  4. 2. Viral dUTPases, Recombinant Expression, Purification and Characterization

    Author : Anna-Carin Bergman; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Proteins; nucleocapsid protein; retrovirus; herpesvirus; dUTPase; nucleotide metabolism; enzymology; Proteiner; enzymologi;

    Abstract : The enzyme deoxyuridine 5’-triphosphate nucleotidohydrolase (dUTPase, EC 3.6.1.23) catalyzes the hydrolysis of dUTP to dUMP and pyrophosphate. READ MORE

  5. 3. On Monomeric and Trimeric dUTPases. Recombinant Expression, Purification, Conformational Properties and Catalytic Characteristics

    Author : Rebecca Persson; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Metabolism; dUTP; dUTPase; nucleotide metabolism; retrovirus; crystallography; herpesvirus; Biokemi; Biochemistry;

    Abstract : Nucleotide metabolism and replication of nucleic acids are processes of fundamental importance. A crucial enzyme in nucleotide metabolism is dUTPase (E.C. 3. READ MORE

  6. 4. Exploiting nucleotide metabolism to improve cancer therapy : by targeting dUTPase, dCTPase and NUDT15

    Author : Anna Hagenkort; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : Synthetic nucleobase- and nucleoside-analogs have stood the test of time and remain a cornerstone in the treatment regimen against various forms of cancer. Due to their resemblance to endogenous nucleotides these antimetabolites interfere with cellular pathways, including nucleotide metabolism, as well as DNA and RNA synthesis. READ MORE

  7. 5. Homotrimeric dUTPases : Principles of Catalysis and Inhibitor Design

    Author : Lorena Gonzalez Palmén; Jan Kvassman; Dolores Gonzalez-Pacanowska; Högskolan i Kalmar; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; dUTPases; kinetics; ground state destabilization; inhibitors; Biochemistry; Biokemi; Natural Science; Naturvetenskap;

    Abstract : The ubiquitous enzyme dUTPase hydrolyzes dUTP into dUMP and pyrophosphate, preventing DNA fragmentation and cell death due to accumulation of dUTP. Inhibitors of dUTPase could serve as drugs in the treatment of cancers and infectious diseases. This thesis presents five studies. READ MORE